Publication: Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar
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Date
2008
Authors
Honda, Jun-ya ; Kobayashi, Leyoshi ; Kiyoshima, Tamotsu ; Yamaza, Haruyoshi ; Xie, Ming ; Takahashi, Keiko ; Enoki, Norio ; Nagata, Kengo ; Nakashima, Akihiko ; Sakai, Hidetaka
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This study examined detailed in situ
expression patterns and possible functional roles of
phosphoglycerate kinase 1 (Pgk1) gene in the
developing tooth germ of the mouse lower first molar.
The strong expression of Pgk1 mRNA was seen in the
odontogenic epithelial cells and surrounding
mesenchymal cells of the tooth germ from embryonic
day 10.5 (E10.5) to E18.0. Western blotting analysis
demonstrated that Pgk1 protein formed 84-kDa protein
complex in these embryonic organs. The results of
immunoprecipitation-western blotting also suggested
this complex to be formed with glyceraldehyde-3-
phosphate dehydrogenase (GAPDH). Moreover, the
immunofluorescence expression of those proteins was
shown to overlap each other in the tooth germ at E15.0.
A strong immunofluorescence expression of both Pgk1
and GAPDH also corresponded to the in situ expression
of those mRNAs. These results suggested that Pgk1
plays some functional roles in the development of tooth
germ and other embryonic organs by forming protein
complex with GAPDH.
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