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Visualizing TGF-ß and BMP signaling in human atherosclerosis: a histological evaluation based on Smad activation

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dc.contributor.authorvan Dijk, R.A.
dc.contributor.authorEngels, C.C
dc.contributor.authorSchaapherder, A.F
dc.contributor.authorMulder-Stapel, A.
dc.contributor.authorten Dijke, P.
dc.contributor.authorHamming, J.F
dc.contributor.authorLindeman, J.H.N.
dc.date.accessioned2017-03-10T17:06:30Z
dc.date.available2017-03-10T17:06:30Z
dc.date.issued2012
dc.description.abstractBackground: The TGF-ß superfamily members Transforming Growth Factor-ß (TGF-ß/Activin) and Bone Morphogenetic Proteins (BMP) have been implicated in the pathogenesis of atherosclerosis. However, their role in human disease remains controversial. In this study we used Smad phosphorylation as a read out for TGF-ß and BMP signaling during the initiation, progression and (de)stabilization of human atherosclerotic disease. Material and methods: A systematic analysis was performed in 114 peri-renal aortic patches (stained with Movat Pentachrome, H&E, pSmad2, pSmad1,5,8 and PAI-1) covering the entire atherosclerotic spectrum (van Dijk, 2010). Immunostaining against T-cells (CD3) and monocytes and macrophages (CD68) was used to explore a putative association between TGF-ß and BMP signaling and vascular inflammation. Results: Smad phosphorylation was present within the normal arterial wall in approximately 10% of the endothelial cells and intimal smooth muscle cells. A significant increase in pSmad2 and pSmad1,5,8 positivity was found in non-progressive lesions (>50% positivity). No further increase or decrease was found in the progressive atherosclerotic lesions, vulnerable and stabilized lesions. No association was found between TGF-ß and BMP signaling and CD3 and CD68 expression, nor cap thickness. Conclusion: Activation of the TGF-ß and BMP pathways is an early event in atherosclerotic lesion formation. No significant relationships were found between Smad phosphorylation and vessel wall inflammation or plaque vulnerability.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationHistology and Histopatholgy. Volume 27, number 3 (March), 2012
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/52417
dc.languageenges
dc.publisherF. Hernandez y JuanF. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología.es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectAtherosclerosises
dc.subjectAortaes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biologíaes
dc.titleVisualizing TGF-ß and BMP signaling in human atherosclerosis: a histological evaluation based on Smad activationes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.27, nº 3 (2012)