Publication: Deletion of GLX3 in Candida albicans affects temperature tolerance, biofilm formation, and virulence
Authors
Cabello, Laura ; Gómez Herreros, Estefanía ; Fernández Pereira, Jordan ; Maicas, Sergi ; Groot, Piet W. J. de ; Valentín, Eulogio ; Martínez-Esparza Alvargonzález, María Concepción
item.page.secondaryauthor
item.page.director
Publisher
Oxford University Press
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1093/femsyr/foy124
item.page.type
info:eu-repo/semantics/article
Description
© FEMS 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Published version of a Published Work that appeared in final form in FEMS Yeast Research. To access the final edited and published work see https://doi.org/10.1093/femsyr/foy124
Abstract
Candida albicans is a predominant cause of fungal infections in mucosal tissues as well as life-threatening bloodstream
infections in immunocompromised patients. Within the human body, C. albicans is mostly embedded in biofilms, which
provides increased resistance to antifungal drugs. The glyoxalase Glx3 is an abundant proteomic component of the biofilm
extracellular matrix. Here, we document phenotypic studies of a glx3 null mutant concerning its role in biofilm formation,
filamentation, antifungal drug resistance, cell wall integrity and virulence. First, consistent with its function as glyoxalase,
the glx3 null mutant showed impaired growth on media containing glycerol as the carbon source and in the presence of low
concentrations of hydrogen peroxide. Importantly, the glx3 mutant showed decreased fitness at 37◦C and formed less
biofilm as compared to wild type and a reintegrant strain. At the permissive temperature of 28◦C, the glx3 mutant showed
impaired filamentation as well as increased sensitivity to Calcofluor white, Congo red, sodium dodecyl sulfate and
zymolyase, indicating subtle alterations in wall architecture even though gross quantitative compositional changes were
not detected. Interestingly, and consistent with its impaired filamentation, biofilm formation and growth at 37◦C, the glx3
mutant is avirulent. Our results underline the role of Glx3 in fungal pathogenesis and the involvement of the fungal wall in
this process.
publication.page.subject
Citation
FEMS Yeast Research. 2019, Vol.. 19, N. 2, foy124
item.page.embargo
Collections
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/