Medium- and long-term Immune responses in the small intestine in piglets from oral vaccination against Escherichia coli

Miralles, Aida; Ramis, Guillermo; Pallarés, Francisco J.; Párraga Ros, Ester; Seva Alcaraz, Juan

Publication:
Medium- and long-term Immune responses in the small intestine in piglets from oral vaccination against Escherichia coli

dc.contributor.author	Miralles, Aida
dc.contributor.author	Ramis, Guillermo
dc.contributor.author	Pallarés, Francisco J.
dc.contributor.author	Párraga Ros, Ester
dc.contributor.author	Seva Alcaraz, Juan
dc.contributor.department	Anatomía y Anatomía Patológica Comparadas
dc.date.accessioned	2024-11-07T13:19:11Z
dc.date.available	2024-11-07T13:19:11Z
dc.date.issued	2024-09-26
dc.description	© 2024 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Animals. To access the final edited and published work see https://doi.org/10.3390/ani14192779	es
dc.description.abstract	Post-weaning stress, together with Escherichia coli, are two of the key factors in the occurrence of post-weaning diarrhea. There are different commercial vaccines that induce immunity at the local or systemic level, improving farm health and avoiding economic losses in the pork industry. That is why the objective of this study was to evaluate the effect of an oral enterotoxigenic E. coli F4/F18 vaccine on immunity and intestinal integrity in the middle and long term after inoculation. The gene expression of the biomarkers indicative of cellular infiltration (calprotectin, CAL), tight junction proteins (occludin, OCL; zonulin, ZON; and claudin, CLA) and a panel of proinflammatory (interleukins, IL: IL1α, IL1β, IL6, IL8, IL12p35 and IL12p40; interferons, IFN: IFNα and IFNγ; and tumoral necrosis factor, TNF: TNFα) and anti-inflammatory mediator cytokines (TGFβ and IL10) were analyzed, as well as histomorphology in jejunum and ileum, the cell density of goblet cells, intraepithelial lymphocytes and IgA-producing cells. Differences were observed in ZON, CLA and CAL, with greater gene expression in observed in vaccinated piglets at 42 days post vaccination (dpv) in the ileum. Regarding the expression of cytokines, the vaccinated animals showed significant differences in IL1α, IL6, IL12p35, IL12p40, IFNα, IFNγ, TNFα and TGFβ at 42 dpv in the jejunum or ileum. The villi showed greater height in the vaccinated piglets and the ratio between villus height and crypt depth was significantly greater in the vaccinated group in the jejunum at 84 dpv. The count of IgA-producing cells shows higher values for the unvaccinated group in the ileum, while intraepithelial lymphocytes show a significant increase in both jejunum and ileum in vaccinated piglets. We can conclude that oral vaccination against E. coli produces an evident effect, which manifests itself even in the middle and long term after the challenge, including immune response, decrease in antimicrobials usage, better histological structure in intestine and the improvement of performance.	es
dc.format	application/pdf	es
dc.format.extent	20	es
dc.identifier.citation	Animals, 2024, Vol. 14, N. 19 : 2779
dc.identifier.doi	https://doi.org/10.3390/ani14192779
dc.identifier.issn	Electronic: 2076-2615
dc.identifier.uri	http://hdl.handle.net/10201/146073
dc.language	eng	es
dc.publisher	MDPI	es
dc.relation	Sin financiación externa a la Universidad	es
dc.relation.publisherversion	https://www.mdpi.com/2076-2615/14/19/2779	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Escherichia coli	es
dc.subject	Oral vaccination	es
dc.subject	Vaccine	es
dc.subject	Intestinal integrity	es
dc.subject	Cytokines	es
dc.subject	Lymphocytes	es
dc.subject	Villus	es
dc.title	Medium- and long-term Immune responses in the small intestine in piglets from oral vaccination against Escherichia coli	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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relation.isAuthorOfPublication.latestForDiscovery	269b9699-1b31-4bb4-82ab-517084bcf737