Publication: Aldehyde dehydrogenase 1B1 is a
potential marker of colorectal tumors
Authors
Wang, Hejing ; Li, Yanmeng ; Zhou, Donghu ; Li, Xiaojin ; Jia, Siyu ; Qi, Saiping ; Huang, Jian
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-304
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info:eu-repo/semantics/article
Description
Abstract
Colorectal cancer (CRC) is common
worldwide. Aldehyde dehydrogenase 1B1 (ALDH1B1),
a member of the ALDH1 family, serves as a biomarker
for cancer stem cells. We hypothesized that ALDH1B1
expression is associated with colorectal tumors.
Immunohistochemistry was used to detect ALDH1B1
expression across a commercial colorectal tissue
microarray. The signal intensities of the positively
stained tissues were expressed using the mean integrated
optical density (mean IOD). We also analyzed
ALDH1B1 mRNA expression in the Oncomine database.
The associations between ALDH1B1 expression and
CRC stage and prognosis were then evaluated using the
web-based tools, GEPIA and UALCAN. Analysis of the
tissue microarray revealed that the expression of
ALDH1B1 was significantly higher in colorectal
adenomas and colorectal adenocarcinoma (IOD/area
values = 0.117±0.070 and 0.168±0.0168, respectively)
compared with normal and cancer-adjacent tissues
(IOD/area values = 0.051±0.028 and 0.068±0.053). For
samples collected in the hospital, ALDH1B1 was highly
expressed in the adenoma (IOD/area = 0.103±0.054) and
CRC (IOD/area = 0.116±0.059) tissues compared with
the cancer-adjacent tissues (IOD/area = 0.066±0.024,
p<0.05). The expression of ALDH1B1 in tissues from
two resources was not found to be significantly
associated with CRC stage. In Oncomine, ALDH1B1
mRNA expression was increased in the colorectal tumor
tissues compared with the normal colorectal tissues
(p=0.024) and its expression was independent of CRC
stage and prognosis (p<0.05). Thus, while the protein
and mRNA expression of ALDH1B1 suggests that it is a
potential marker of colorectal tumors, its expression is
independent of CRC stage and prognosis.
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Citation
Histology and Histopathology Vol. 36, nº2 (2021)
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