Publication: Modulation of the glycoconjugate
expression in the tracheo-bronchial
epithelium during sustained hypovitaminosis A
Authors
Zschabitz, A. ; Weiser, H. ; Stofft, E. ; Gabius, H.J. ; Biesalsk, H. K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim of this study was to determine the
influence of sustained marginal vitamin A deficiency
on the morphology of glycoconjugate expression
in the tracheobronchial epithelium of guinea pigs.
The distribution of oligosaccharide chains was
investigated by applying a panel of 24 lectins.
Glycosaminoglycans were detected by histochemical
techniques. Number as well as morphology of ciliated
cells showed no significant alterations in hypovitaminosis
A. In contrast, the quantity of goblet
cells was constantly decreased. A considerable reduction
of secretory granules was also observed in these cells.
Cytomembranes of ciliated cells (especially in the area
of ciliar extensions) showed constant alterations in the
patterns of lectin binding in vitamin A-depleted guinea
pigs. Our results demonstrate a significant augmentation
of accessibility of fucosyl molecules in proximal
domains of glycoconjugates of ciliary membranes,
whereas the presence of mannose structures seemed
unchanged. In dista1 bronchioli, terminal Nacetylgalactosamine
molecules were expressed. During
marginal vitamin A deficiency, ciliary cells were
specially labelled by GSA IB, indicating presentation of
terminal galactose molecules in a-position. Additionally,
the cytoplasm of epithelial cells demonstrated enhanced
concentrations of polyantennary oligosaccharide core
structures. Staining of epithelial cells by VVA was
restricted to control specimens. Abundance of Nacetylglucosamine
residues on the non-reducing
terminus of oligosaccharides was significantly enhanced
in the connective tissue of depleted animals
as demonstrated by the binding patterns of GSA 11.
We suggest that altered oligosaccharide patterns
may contribute to enhanced predisposition to
tracheobronchial infection in marginal vitamin A
deficiency.
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