Publication:
Use of b-cyclodextrins to enhance phytosterol production in cell suspension cultures of carrot (Daucus carota L.)

Simple item page
dc.contributor.authorPedreño García, María Ángeles
dc.contributor.authorSabater Jara, Ana Belén
dc.contributor.departmentBiología Vegetal
dc.date.accessioned2025-05-15T10:02:38Z
dc.date.available2025-05-15T10:02:38Z
dc.date.issued2013-04-14
dc.description© 2013 Springer Science+Business Media Dordrecht. This document is the Published Manuscript version of a Published Work that appeared in final form in Plant Cell, Tissue and Organ Culture. To access the final edited and published work see https://doi.org/10.1007/s11240-013-0320-4
dc.description.abstractPhytosterols are isoprenoid-derived compounds that play essential roles in plant growth and development as they are integral components of the plant cell membranes, and responsible for their permeability and fluidity. In this study, the effect of different types of beta-cyclodextrins (β-CD) on phytosterol production was evaluated using Daucus carota cell suspension cultures. A detailed analysis provides the optimal type and concentration of β-CD, elicitation time, and the optimal cell age and density. The highest levels of phytosterols produced by cells and secreted to the culture medium were obtained when 10 day-old D. carota cell suspension cultures, with an initial cell density of 200 g of fresh weight (FW) l−1, were incubated in the presence of 50 mM of methylated-β-CD (M-β-CD) for 144 h. In addition, M-β-CD significantly promoted the accumulation of phytosterol in the extracellular medium of D. carota cell suspension cultures, which was not observed in control cell suspension cultures. Moreover, these high phytosterol levels did not improve when methyl jasmonate (MJ) was added to the cell suspension cultures alone or combined with 50 mM M-β-CD, although MJ stimulated the formation of defense-related compounds. Therefore, the use of carrot cell suspension cultures seems a promising biotechnological alternative since the addition of β-CD to the culture medium not only induced the biosynthesis of phytosterols but also promoted their secretion into the culture medium, where they were accumulated and could be isolated easily.
dc.formatapplication/pdfes
dc.format.extent10
dc.identifier.citationPlant Cell, Tissue and Organ Culture, 2013, Vol. 114, pp. 249–258
dc.identifier.doihttps://doi.org/10.1007/s11240-013-0320-4
dc.identifier.issnPrint: 0167-6857
dc.identifier.issnElectronic:1573-5044
dc.identifier.urihttp://hdl.handle.net/10201/154693
dc.languageenges
dc.publisherSpringer
dc.relationFundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia (08799/PI/08), MICINN-FEDER (BIO2008-2941 y BIO2011-29856-C02-02).es
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s11240-013-0320-4
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCell suspension cultures
dc.subjectCyclodextrin
dc.subjectDaucus carota L
dc.subjectPhytosterol
dc.titleUse of b-cyclodextrins to enhance phytosterol production in cell suspension cultures of carrot (Daucus carota L.)es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication6a60e21b-276a-41c1-9f7e-16b7c933da77
relation.isAuthorOfPublication4c6e7506-0db4-477e-8704-2a1783040719
relation.isAuthorOfPublication.latestForDiscovery6a60e21b-276a-41c1-9f7e-16b7c933da77
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2013_Sabater-Jara_Use of b-cyclodextrins to enhance phytosterol production.pdf
Size:
491.45 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos