The impact of in vitro embryo production on placental and umbilical cord vascularization is minimized by the addition of reproductive fluids

Álvarez Martín, Úrsula; Coy, Pilar; Romar, Raquel; Párraga Ros, Ester; Seva Alcaraz, Juan

Publication:
The impact of in vitro embryo production on placental and umbilical cord vascularization is minimized by the addition of reproductive fluids

dc.contributor.author	Álvarez Martín, Úrsula
dc.contributor.author	Coy, Pilar
dc.contributor.author	Romar, Raquel
dc.contributor.author	Párraga Ros, Ester
dc.contributor.author	Seva Alcaraz, Juan
dc.contributor.department	Anatomía y Anatomía Patológica Comparadas
dc.date.accessioned	2024-11-07T13:18:54Z
dc.date.available	2024-11-07T13:18:54Z
dc.date.issued	2023-09-15
dc.description	© 2023 The Authors. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published version of a Published Work that appeared in final form in Theriogenology. To access the final edited and published work see https://doi.org/10.1016/j.theriogenology.2023.05.029	es
dc.description.abstract	Animals born from in-vitro-produced (IVP) embryos show changes in the placenta and umbilical cord vascularization. This study compares the placental and umbilical vascular morphometry in pigs (n = 19) born through artificial insemination (AI group) or after transfer of IVP embryos cultured with (RF-IVP group) or without (C-IVP group) reproductive fluids. The relationship between vascular parameters and animal growth during the first year of life was also analyzed. Samples were collected at birth, fixed, paraffin-embedded, cut in sections, stained, and photographed for vascular and morphometric analysis with ImageJ® and Slide Viewer®. The average daily weight gain was individually scored from birth to the first year of life. No differences were found in placental vascular morphometry among groups, except for the vascular area of small vessels (arterioles, venules, and small vessels) that was higher in the C-IVP group. Regarding the umbilical cord, the values for perimeter (AI: 26.40 ± 3.93 mm; IVP: 30.51 ± 4.74 mm), diameter (AI: 8.35 ± 1.01 mm; IVP: 10.26 ± 1.85 mm), area (AI: 43.18 ± 12.87; IVP: 56.61 ± 14.89 mm2), and Wharton's jelly area (AI: 36.86 ± 12.04 mm2; IVP 48.88 ± 12.80 mm2) were higher in IVP-derived than AI-derived animals, whereas arterial and venous morphometric data were similar between groups. A correlation study showed that placental and umbilical cord vascular phenotypes affect the further growth of pigs. In conclusion, assisted reproductive technologies impact small caliber vessels in the placenta and morphometric parameters in the umbilical cord. The addition of reproductive fluids in IVP-embryo contributes to reduce the differences with in vivo-derived animals.	es
dc.format	application/pdf	es
dc.format.extent	9	es
dc.identifier.citation	Theriogenology, 2023, Vol. 208, pp. 149-157
dc.identifier.doi	https://doi.org/10.1016/j.theriogenology.2023.05.029
dc.identifier.issn	Electronic: 1879-3231
dc.identifier.issn	Print: 0093-691X
dc.identifier.uri	http://hdl.handle.net/10201/146072
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	This research was funded by MCIN/AEI/10.13039/501100011033/and “FEDER Una manera de hacer Europa”, grant number I + D + i PID2020-113366RB-I00.	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0093691X23001991?via%3Dihub	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Placenta	es
dc.subject	Umbilical cord	es
dc.subject	Vascularization	es
dc.subject	ART	es
dc.subject	Reproductive fluids	es
dc.subject	Growth	es
dc.subject	Pigs	es
dc.title	The impact of in vitro embryo production on placental and umbilical cord vascularization is minimized by the addition of reproductive fluids	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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