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A novel CD14high CD16high subset of peritoneal macrophages from cirrhotic patients is associated to an increased response to LPS

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dc.contributor.authorFernández-Fernández, María Dolores
dc.contributor.authorTristán-Manzano, María
dc.contributor.authorSánchez-Velasco, Eduardo
dc.contributor.authorMiras-López, Manuel
dc.contributor.authorGarcía-Penarrubia, Pilar
dc.contributor.authorHernández Caselles, Trinidad
dc.contributor.authorMartínez-Esparza Alvargonzález, María Concepción
dc.contributor.authorRuiz Alcaraz, Antonio José
dc.contributor.authorTapia Abellán, Ana
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2024-07-02T07:53:31Z
dc.date.available2024-07-02T07:53:31Z
dc.date.issued2016-03-01
dc.description© 2016 Elsevier Ltd. All rights reseved. This document is the Published version of a Published Work that appeared in final form in Molecular Immunology. To access the final edited and published work see https://doi.org/10.1016/j.molimm.2016.02.012
dc.description.abstractThe aim of this study was to characterize monocyte-derived macrophages (M-DM) from blood and ascites of cirrhotic patients comparatively with those obtained from blood of healthy controls. The phenotypic profile based on CD14/CD16 expression was analyzed by flow cytometry. Cells were isolated and stimulated in vitro with LPS and heat killed Candida albicans. Phosphorylation of ERK, c-Jun, p38 MAPK, and PKB/Akt was analyzed by Western blotting. A novel CD14(high)CD16(high) M-DM subpopulation is present in ascites (∼33%). The CD14(++)CD16(+) intermediate subset is increased in the blood of cirrhotic patients (∼from 4% to 11%) and is predominant in ascites (49%), while the classical CD14(++)CD16(-) subpopulation is notably reduced in ascites (18%). Basal hyperactivation of ERK and JNK/c-Jun pathways observed in ascites M-DM correlates with CD14/CD16 high expressing subsets, while PI3K/PKB does it with the CD16 low expressing cells. In vitro LPS treatment highly increases ERK1/2, PKB/Akt and c-Jun phosphorylation, while that of p38 MAPK is decreased in M-DM from ascites compared to control blood M-DM. Stimulation of healthy blood M-DM with LPS and C. albicans induced higher phosphorylation levels of p38 than those from ascites. Regarding cytokines secretion, in vitro activated M-DM from ascites of cirrhotic patients produced significantly higher amounts of IL-6, IL-10 and TNF-α, and lower levels of IL-1β and IL-12 than control blood M-DM. In conclusion, a new subpopulation of CD14(high)CD16(high) peritoneal M-DM has been identified in ascites of cirrhotic patients, which is very sensitive to LPS stimulation.es
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dc.format.extent9es
dc.identifier.citationMolecular Immunology 72 (2016) 28–36
dc.identifier.doihttps://doi.org/10.1016/j.molimm.2016.02.012
dc.identifier.issnPrint: 0161-5890
dc.identifier.issnElectronic: 1872-9142
dc.identifier.urihttp://hdl.handle.net/10201/142789
dc.languageenges
dc.publisherElsevieres
dc.relationThis study was funded with a grant from the Fundación Séneca, Comunidad Autónoma de la Región de Murcia (Project grant no: 11926/PI/09).es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0161589016300256?via%3Dihubes
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCirrhosises
dc.subjectAsciteses
dc.subjectInflammationes
dc.subjectMonocyte subsetses
dc.subjectCell signalinges
dc.subjectCytokineses
dc.titleA novel CD14high CD16high subset of peritoneal macrophages from cirrhotic patients is associated to an increased response to LPSes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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