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Chondrocyte differentiation for auricular cartilage reconstruction using a chitosan based hydrogel

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dc.contributor.authorGarcía-López, J.
dc.contributor.authorGarciadiego-Cázares, D.
dc.contributor.authorMelgarejo-Ramírez, Y.
dc.contributor.authorSánchez-Sánchez, R.
dc.contributor.authorSolís-Arrieta, L.
dc.contributor.authorGarcía-Carvaja, Z.
dc.contributor.authorSánchez-Betancourt, J.I.
dc.contributor.authorIbarra, C.
dc.contributor.authorLuna-Bárcena, G.
dc.contributor.authorVelasquillo, C.
dc.date.accessioned2021-02-22T08:15:13Z
dc.date.available2021-02-22T08:15:13Z
dc.date.issued2015
dc.description.abstractTissue engineering with the use of biodegradable and biocompatible scaffolds is an interesting option for ear repair. Chitosan-Polyvinyl alcohol-Epichlorohydrine hydrogel (CS-PVA-ECH) is biocompatible and displays appropriate mechanical properties to be used as a scaffold. The present work, studies the potential of CS-PVA-ECH scaffolds seeded with chondrocytes to develop elastic cartilage engineered-neotissues. Chondrocytes isolated from rabbit and swine elastic cartilage were independently cultured onto CS-PVA-ECH scaffolds for 20 days to form the appropriate constructs. Then, in vitro cell viability and morphology were evaluated by calcein AM and EthD-1 assays and Scanning Electron Microscopy (SEM) respectively, and the constructs were implanted in nu/nu mice for four months, in order to evaluate the neotissue formation. Histological analysis of the formed neotissues was performed by Safranin O, Toluidine blue (GAG’s), Verhoeff-Van Gieson (elastic fibers), Masson’s trichrome (collagen) and Von Kossa (Calcium salts) stains and SEM. Results indicate appropriate cell viability, seeded with rabbit or swine chondrocyte constructs; nevertheless, upon implantation the constructs developed neotissues with different characteristics depending on the animal species from which the seeded chondrocytes came from. Neotissues developed from swine chondrocytes were similar to auricular cartilage, while neotissues from rabbit chondrocytes were similar to hyaline cartilage and eventually they differentiate to bone. This result suggests that neotissue characteristics may be influenced by the animal species source of the chondrocytes isolated.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.citationHistology and Histopathology, vol. 30, nº12, (2015)
dc.identifier.doi10.14670/HH-11-642
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/103641
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectAuricular cartilagees
dc.subjectMicrotiaes
dc.subjectChondrocyte differentiationes
dc.subjectChitosan hydrogeles
dc.subjectEndochondral ossificationes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleChondrocyte differentiation for auricular cartilage reconstruction using a chitosan based hydrogeles
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30,nº12 (2015)