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In vitro cytotoxicity assessment of monocationic and dicationic pyridinium-based ionic liquids on HeLa, MCF-7, BGM and EA.hy926 cell lines

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dc.contributor.authorPérez, S. A.
dc.contributor.authorGarcía Montalbán, Mercedes
dc.contributor.authorCarissimi, Guzmán
dc.contributor.authorLicence, P.
dc.contributor.authorVíllora Cano, Gloria
dc.contributor.departmentIngeniería Química
dc.date.accessioned2025-01-31T08:20:49Z
dc.date.available2025-01-31T08:20:49Z
dc.date.issued2019-10-24
dc.description© 2019 Elsevier B.V. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Journal of Hazardous Materials. To access the final edited and published work see https://doi.org/10.1016/j.jhazmat.2019.121513
dc.description.abstractDicationic ionic liquids (ILs) generally possess higher thermal and electrochemical stability than the analogous monocationic ILs, which makes them more suitable for high-temperature applications as solvents for organic reactions, lubricants or stationary phase in gas chromatography. However, knowledge on dicationic IL cytotoxicity is still scarce. Here we explore the cytotoxicity of twelve mono- and dicationic pyridinium-based ILs on HeLa, MCF-7, BGM and EA.hy926 cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell cycle arrest assays, apoptosis experiments and orange staining were carried out. The results showed that dicationic ILs are generally less cytotoxic than their monocationic counterparts. In monocationic ILs, cytotoxicity was stronger when they contain long alkyl chains, because of their higher lipophilicity. However, the full effect of the length of the linkage alkyl chain of dicationic ILs on cytotoxicity is not clear probably because the chain is “trapped” between both cationic moieties. IL cytotoxicity is highly dependent on the cell type, and HeLa cells exposed to [C12Pyr]Br die via apoptosis. The present study increases our knowledge of IL cytotoxicity on human and monkey cells and clarifies the cell death mechanism. The results suggest that dicationic ILs offer the potential to replace some monocationic ILs because of their lower cytotoxicity.es
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dc.format.extent9es
dc.identifier.citationJournal of Hazardous Materials, 385 (2020) 12151
dc.identifier.doihttps://doi.org/10.1016/j.jhazmat.2019.121513
dc.identifier.issnPrint: 0304-3894
dc.identifier.issnElectronic: 1873-3336
dc.identifier.urihttp://hdl.handle.net/10201/149825
dc.languageenges
dc.publisherElsevieres
dc.relationThis work was partially supported by the European Commission (FEDER/ERDF) and the Spanish MINECO (Ref. CTQ2017-87708-R) and the research support programme of the Seneca Foundation of Science and Technology of Murcia, Spain (Ref. 20977/PI/18). M. G. Montalbán acknowledges support from MINECO (Juan de la Cierva-Formación contract, Ref. FJCI-2016-28081), P. Licence acknowledges EPSRC and BBSRC for support (EP/S005080/1, EP/P013341/1, BB/L013940/1).es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0304389419314670?via%3Dihub
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectDicationices
dc.subjectIonic liquides
dc.subjectPyridiniumes
dc.subjectMTT assayes
dc.subjectApoptosises
dc.titleIn vitro cytotoxicity assessment of monocationic and dicationic pyridinium-based ionic liquids on HeLa, MCF-7, BGM and EA.hy926 cell lineses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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