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Human keratinocytes cultured without a feeder layer undergo progressive loss of differentiation markers

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dc.contributor.authorPrignano, F.es
dc.contributor.authorDomenici Lombardo, L.es
dc.contributor.authorGerlini, G.
dc.contributor.authorPimpinelli, N.
dc.contributor.authorRomagnoli, P.
dc.date.accessioned2011-02-22T11:09:40Z
dc.date.available2011-02-22T11:09:40Z
dc.date.issued1999
dc.description.abstractCulture of keratinocytes in conventional medium without a mesenchyme-derived feeder layer leads to poor growth and impaired differentiation; however, the exact pathway and degree of differentiation achieved in such conditions is unclear. We have cultured normal human keratinocytes in Rheinwald and Green's medium, on plastic without a feeder layer, in order to investigate the degree of differentiation that they achieve in these conditions. Intermediate filament proteins, tonofibrils and desmosomes were assumed as markers of differentiation and their expression was analyzed by immunohistochemistry and electron micros~opy. Before reaching confluence, keratinocytes expressed keratin molecules, as well as vimentin, and formed tonofibrils and desmosomes. The expression of these markers was progressively reduced until confluence and was totally lost thereafter, while cultures could be propagated for at least six passages. On the contrary, reseeding on a feeder layer after the first passage led to rapid cell death. It could be concluded that signals from a feeder layer are relevant to support continuous synthesis of intermediate filaments proteins and formation of tonofibils and desmosomes, and that the derangement of the cytoskeleton in these conditions leads to altered, not simply defective, response to delayed stimulation by a feeder layer.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/19225
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyen_EN
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectDesmosomeen_EN
dc.subjectKeratinsen_EN
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.5 - Piel. Dermatología clínicaes
dc.titleHuman keratinocytes cultured without a feeder layer undergo progressive loss of differentiation markersen_EN
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.14, nº 3 (1999)