Publication: Sex steroid determination during periestrous and peridiestrous period in saliva of sows derived from assisted reproductive techniques
Authors
Navarro, Elena ; Heras, Sonia ; Quintero-Moreno, Armando A. ; Romar, Raquel ; Romero Aguirregomezcorta, Jon
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Publisher
Brazilian College of Animal Reproduction
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DOI
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info:eu-repo/semantics/lecture
Description
Abstract
Emerging evidence indicates a long-lasting effect of in vitro fertilization on molecular physiology and metabolic dysfunction (Feuer and Rinaudo, J Dev Orig Health Dis 8, 2017). In pigs, clear evidence of phenotypical differences between artificial insemination and in vitro-derived animals have been reported (París-Oller et al., Res Vet Sci 142, 2021; Paris-Oller et al., J Dev Orig Health Dis 13, 2022). These differences
might be extended to reproductive phenotype. Therefore, the reproductive stage and the concentrations of 17ß-oestradiol (E2) and progesterone (P4) were monitored in saliva samples of 4-year-old sows obtained from a previous study (Paris-Oller et al., J Anim Sci Biotech 12, 2021). Animals were born after artificial insemination (AI group; n=8) and surgical transfer of in vitro-produced embryos (IVP group; n=12), housed in
an open pen and fed under the same conditions. Sows’ interest in the male was recorded by a boar station (Compident) that registered the number and duration of visits to the station. Simultaneously, sows were subjected to the back pressure test. Estrus (d1 estrous cycle) was defined as the day of longer residence time at the station together with immobilization response to the back pressure test. Saliva samples were
collected using Salivette® tubes containing the polystyrene sponge previously chewed by the sows for 10 seconds. Tubes were centrifuged (1.000 g, 5 min) and samples stored (-80ºC) until hormone analysis by quimioluminescence (Immulite). Concentration of E2 and P4 was determined in samples collected from days 1-3 (periestrous period; PEP) and 13-15 (peridiestrous period, PDP). Data (mean±SD) for number of visits to boar station (n), duration of the visits (sec), E2 (pg/ml) and P4 (ng/ml) were normalized and then analyzed by one-way repeated-measurements ANOVA followed by uncorrected Fisher’s to compare variables between groups. P < 0.05 was considered significant. During PEP, sows from IVP group visited the boar station more times (10.60±1.91 vs. 3.30±1.05) and for longer time (239.77±43.12 secs vs. 77.69±26.01 secs) than AI sows. This observation is attributed to the higher hierarchy of IVP animals in this herd. No differences were found in E2 concentration (108.03±2.50 pg/ml and 106.81±4.95 pg/ml, respectively for IVP and AI animals). However, P4 concentration was higher in IVP (0.23±0.03 ng/ml) than AI sows (0.12±0.01 ng/ml). During PDP, only IVP sows visited the boar station (10.80±8.60 times for 265.0± 213.13 secs) and no
differences between groups were found in E2 concentration (121.16±5.91 pg/ml and 119.11±3.40 pg/ml) neither P4 (0.21±0.06 ng/ml and 0.32±0.05 ng/ml; respectively for AI and IVP sows). Surprisingly, E2 levels in saliva were higher during PDP than PEP in IVP-derived animals but not in AI-derived animals (P=0.06). As for P4, no differences were found between concentration during PEP and PDP in any group of animals. Further analysis of saliva samples collected from whole estrous cycle will help to interpretation of data.
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Citation
Anim Reprod. 2023; 20(2 )
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