Publication: Remote lung injury after experimental
intestinal ischemia-reperfusion in horses
Authors
Montgomery, Julia B. ; Hamblin, Brie ; Suri, Sarabjeet Singh ; Johnson, Laura E. ; New, Dallas ; Johnston, Jennifer ; Kelly, Jenny ; Wilson, David G. ; Singh, Baljit
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Ischemia followed by reperfusion leads to
release of toxic molecules into the circulation, and these
molecules may cause injury in remote organs such as the
lung. Horses commonly suffer from episodes of
intestinal ischemia-reperfusion (IR) due to intestinal
twisting/strangulation followed by repair. Because there
is no evidence of lung injury associated with IR in
horses, we designed a study to characterize the intestinal
IR-associated lung inflammation and determine the
effect of lidocaine on lung inflammation in IR horses.
Lung tissues were collected from non-anesthetized (n=4)
and anesthetized (n=4) control horses and horses (n=12)
after 70 minutes of ischemia followed by 60 minutes of
reperfusion. Horses in IR groups received Lactated
Ringer’s Solution (LRS; n=6) or lidocaine (n=6)
intravenously. Control lungs had normal histology but
lungs from IR horses showed moderate accumulation of
neutrophils in blood vessels and airways. We found
increased staining for TLR4, IL-8, TLR9, and von
Willebrand factor (vWF) along with aggregates of vWFpositive
platelets in lung vessels of IR horses compared
to the controls. Lung TNFα was significantly increased
in IR horses compared to the control horses (P<0.05).
Neutrophil numbers, but not MPO concentrations, were
significantly lower, while macrophage numbers were
higher in the IR group receiving lidocaine compared to
the LRS horses (P<0.05). We conclude that intestinal IR
leads to remote lung injury characterized by recruitment
of inflammatory cells and expression of inflammatory
molecules in horses, and lidocaine may ameliorate lung
inflammation following intestinal IR.
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Citation
Histology and Histopathology, vol. 29, nº 3 (2014)
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