Publication: The nuclear matrix: a critical appraisal
Authors
Martelli, A.M. ; Cocco, L. ; Riederer, B.M. ; Neri, L.M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
It is becoming increasingly clear that the cell
nucleus is a highly structurized organelle. Because of its
tight compartmentalization, it is generally believed that a
framework must exist, responsible for maintaining such
a spatial organization.
Over the last twenty years many investigations have
been devoted to identifying the nuclear framework.
Structures isolated by different techniques have been
obtained in vitro and are variously referred to as nuclear
matrix, nucleoskeleton or nuclear scaffold. Many
different functions, such as DNA replication and repair,
mRNA transcription, processing and transport have been
described to occur in close association with these
structures. However, there is still much debate as to
whether or not any of these preparations corresponds to
a nuclear framework that exists in vivo. In this article we
summarize the most commonly-used methods for
obtaining preparations of nuclear frameworks and we
also stress the possible artifacts that can be created in
vitro during the isolation procedures.
Emphasis is placed also on the protein composition
of the frameworks as well as on some possible signalling
functions that have been recently' described to occur in
tight association with the nuclear matrix.
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