Publication: The immunohistochemical expression of
stress-response protein (srp) 60 in human brain
tumours: Relationship of srp 60 to the other five
srps, proliferating cell nuclear antigen and p53 protein
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Date
2001
Authors
Kato, S. ; Kato, Massuo J. ; Hirano, A. ; Takikawa, M. ; Ohama, E.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This study analyzed the expression of stressresponse
(heat-shock) protein 60 (srp 60) in a series of
158 human brain tumours. Immunohistochemical
procedures were employed; cells of the human cervical
cancer line HeLa S3 exposed to hyperosmolar stress
served as positive controls. Deposits of reaction products
were found in the cytoplasm. Approximately half of the
glioblastomas multiforme (17/31), breast carcinoma
metastases (6/10), and lung carcinoma metastases (5111)
as well as about one-third of the astrocytomas (5113) and
meningiomas (8123) had tumour cells that expressed srp
60. A positive reaction for srp 60 was also seen in some
medulloblastomas (2/16), primitive neuroectodermal
tumours (PNETs) (2/11), schwannomas (2121), and
pituitary adenomas (2/7), but no positive reactions were
observed with oligodendrogliomas and ependymomas.
Compared with srp 60-negative tumours, srp 60-positive
tumours coexpressed one or more stress-related proteins,
among which srp 90, srp 72, srp 27, alphaB-crystallin
and ubiquitin occurred with higher frequencies; a high
correlation between srp 60 and the other five srps (0.88 -
0.97, pe0.01, Pearson correlation coefficient) was
observed in srp 60-positive tumours. In contrast, the
correlation coefficient in srp 60-negative tumours was
not significant (-0.26 - 0.71). There was a tendency for
the proliferating cell nuclear antigen (PCNA)-labeling
index to be higher in glioblastomas, astrocytornas,
medulloblastomas, PNETs, and breast and lung
carcinoma metastases that expressed srp 60 than in those
that did not. No significant immunohistochemical
reactions of srp 60, PCNA and p53 protein were seen
with sections of normal brain tissues. We conclude that
primary and metastatic tumours of the brain produce srp
60 and that srp 60 in certain brain tumour cells may coexpress the other five srps. In addition, srp 60
expression might depend, in part, on proliferating
potential.
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