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Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?

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dc.contributor.authorLi, Junwei
dc.contributor.authorRoca Aleu, Jorge
dc.contributor.authorPérez Patiño, Cristina
dc.contributor.authorBarranco Cascales, Isabel
dc.contributor.authorMartínez García, Emilio
dc.contributor.authorRodríguez Martínez, Heriberto
dc.contributor.authorParrilla Riera, Inmaculada
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2025-10-16T11:54:37Z
dc.date.available2025-10-16T11:54:37Z
dc.date.copyright© 2018 Elsevier B.V. All rights reserved
dc.date.issued2018-08
dc.description.abstractThis study aimed to elucidate the effect of seminal plasma (SP) from post-SRF on boar sperm freezability and, in addition, to determine the relevance of sperm itself to sustain cryopreservation, regardless of the SP surrounding them. Twelve ejaculates from three boars were manually collected in fractions/portions, P1: the first 10 mL of the SRF, P2: the rest of the SRF and the post-SRF. Immediately, samples were centrifuged to separate spermatozoa from the surrounding SP. Spermatozoa from P1 and P2 were then incubated with its own SP or that from post-SRF, diluted in BTS (1:1, v/v) at 17 °C overnight before being frozen in 0.5 mL straws using a standard protocol. Sperm motility (total and progressive) deteriorated (P < 0.05) when P1- or P2-sperm when incubated overnight in SP from post-SRF, while sperm viability differed between P1 and P2 (P < 0.05) regardless of the SP they were incubated in. Post-thaw sperm quality and functionality differed between P1 and P2, regardless of the SP used for overnight pre-freezing incubation. Post-thaw motility (P < 0.05) and viability (P < 0.01), as well as plasma membrane fluidity (P < 0.05) or lipid peroxidation values (P < 0.01) were best in P1 sperm compared to those of P2. The protein profile of sperm from P1 and P2, analyzed by 2D-PAGE, showed qualitative differences, which suggest that sperm rather than SP would explain differences in sperm freezability between ejaculate fractions/portions. Use of P1 fraction spermatozoa seems thus optimal for cryopreservation.
dc.formatapplication/pdf
dc.format.extent8
dc.identifier.citationAnimal Reproduction Science, 2018, Vol. 195, pp. 30-37
dc.identifier.doihttps://doi.org/10.1016/j.anireprosci.2018.05.002
dc.identifier.eissn0378-4320
dc.identifier.issn0378-4320
dc.identifier.urihttp://hdl.handle.net/10201/166810
dc.languageeng
dc.publisherElsevier
dc.relationThe authors are grateful to AIM Iberica (Topigs Norsvin España) for providing the semen samples. The study was supported by Seneca Foundation Murcia, Spain (19892/GERM-15) and the Swedish Research Council Formas (2017-00946), Stockholm, Sweden. Junwei Li was financially supported by China Scholarship Council (2013/3009).
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0378432017309363
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBoar
dc.subjectEjaculate portions
dc.subjectSeminal plasma
dc.subjectSperm cryopreservation
dc.subjectSperm protein profile
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleIs boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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