Publication:
Type 1 diabetes mellitus induced low bone turnover in ovariectomized rats

Thumbnail Image
Files
Liu-34-57-67-2019.pdf(11.42 MB)
Date
2019
relationships.isAuthorOfPublication
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
Liu, Bo ; Feng, Wei ; Hasegawa, Tomoka ; Amizuka, Norio ; Li, Minqi
item.page.secondaryauthor
item.page.director
Publisher
publication.page.editor
publication.page.department
DOI
DOI: 10.14670/HH-18-024
item.page.type
info:eu-repo/semantics/article
Description
Abstract
To investigate type 1 diabetes mellitus (T1DM) affecting bone remodeling in the context of menopause in female rats. The animals were subjected to either ovariectomy (OVX) which was performed to mimic postmenopausal estrogen deficiency, and/or type 1 diabetes mellitus which was established by the intraabdominal administration of 50 mg/kg streptozotocin (STZ). Single-loaded groups were the OVX group and the STZ group, while the combined group was the OVX+STZ group. Bone histomorphometry was performed on the tibial metaphysis by Micro-CT scanning. Immunohistochemistry was used to assess the activity of osteoblast and osteoclast by counteracting with antibodies against their respective specific marker enzymes. The gene expression of key molecules involved in osteoblastic and osteoclastic signaling pathways were analyzed by RT-qPCR. The results showed a significant bone volume decrease in both single-loaded groups and combined group with the combined group suffering greatest bone loss and bone structural deterioration (p<0.001). Immunohistochemical staining and RT-qPCR revealed an increase in osteoblastic (p<0.001) and osteoclastic (p<0.01) activities in OVX rats while there was a decrease (p<0.05) in those of STZ rats. When OVX and STZ were combined, the rats exhibited a further decrease in osteoblastic activity (p<0.001) and a similar level of osteoclastic activity (p>0.05) compared to their STZ counterparts. These results demonstrated that STZinduced T1DM reverses OVX-associated high bone turnover osteoporosis to the type of low bone turnover, leading to greater bone loss and structural defect.
publication.page.subject
Type 1 , diabetes mellitus , Bone turnover , Osteoporosis
Citation
Histology and Histopathology, Vol.34, nº1, (2019)
URI
http://hdl.handle.net/10201/120944
item.page.embargo
Collections
Histology and histopathology Vol.34, nº1 (2019)
Ir a Estadísticas