Publication: Effect of rehabilitation protocols on muscle function and morphology following hindlimb disuse in weanling rats
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Date
2016
Authors
Leite Nogut, Keite i ; Bianchi, Eduardo ; Chesca Simões, Deise Lúcia ; Mattiello-Sverzut, Ana Claudia ; de Moura-Jucá, Renata Viana Brígido
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
10.14670/HH-11-653
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info:eu-repo/semantics/article
Description
Abstract
Background: Primary or secondary disorders
in developing skeletal muscles are prevalent in physical
therapy practice. Assessment of gait functional changes
and morphological aspects of hindlimb muscles of
weanling rats have not been reported simultaneously in
the literature. Rehabilitation by active (eccentric
training) and passive (stretching) exercises after
hypomobility needs to be investigated. Methods: After
ten days of immobilisation in a plantar flexion-shortened
position, animals underwent eccentric training on
treadmills, intermittent (a single series of ten exercises
of 30 seconds each, with a 30-s interval) or continuous
stretching protocols for 40 minutes, or had free cage
activity for three days. Analysis of gait variables and
muscle morphology (immunohistochemical staining of
soleus and plantar muscles for fibronectin and types I
and III collagen and immunofluorescence staining for
dystrophin, laminin, Pax-7, and CD68) were performed.
Results: On the third day, the rehabilitated animals
touched the ground surface with their toes, except for the
group undergoing continuous stretching. The total
amount of extracellular macrophages was higher in the
rehabilitated animals. The number of satellite cells was
not significantly different between groups. Conclusion:
Three days of active training (eccentric exercise) showed
greater effectiveness compared to the other rehabilitation
programs. Weanling rats seem to respond differently to
external stimuli such as disuse and remobilisation.
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Citation
Histology and Histopathology, vol.31, nº 2, (2016)
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