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Insulin degrading enzyme is up-regulated in pancreatic β cells by insulin treatment

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dc.contributor.authorFernández Díaz, Cristina M.
dc.contributor.authorEscobar Curbelo, Luis
dc.contributor.authorLópez Acosta, J.F.
dc.contributor.authorLobatón, Carmen D.
dc.contributor.authorMoreno, Alfredo
dc.contributor.authorSanz Ortega, Julián
dc.contributor.authorPerdomo, Germán
dc.contributor.authorCózar Castellano, Irene
dc.date.accessioned2022-05-27T11:21:49Z
dc.date.available2022-05-27T11:21:49Z
dc.date.issued2018
dc.description.abstractInsulin Degrading Enzyme (IDE) is an endopeptidase that degrades insulin and glucagon. Ide gene has been associated with type-2 diabetes mellitus (DM2). However, the physiological role(s) of IDE in glucose homeostasis and its potential therapeutic benefit remain not completely known. To contribute in the understanding of IDE's role in glucose metabolism, we analyzed IDE protein level in pancreatic islets from two hyperinsulinemic mouse models, db/db and high-fat diet (HFD) mice, as well as in human islets from DM2 patients treated with oral hypoglycemic agents (OHAs) or insulin. IDE protein level was detected by staining and by western-blot. INS1E cells, rat and human islets were treated with insulin and IDE protein level was studied. We have shown for the first time IDE staining in rodent and human tissue, using the proper negative control, IDE null mouse tissue. Our staining indicates that IDE is expressed in both beta- and alpha-cells, with higher expression in alpha-cells. Db/db and HFD mice islets showed increased IDE protein level. Interestingly, human islets from DM2 patients treated with OHAs showed decreased IDE protein level in beta-cells. Meanwhile, islets from insulin-treated DM2 patients showed augmented IDE protein level compared to OHAs patients, pointing to an upregulation of IDE protein level stimulated by insulin. These data correlate nicely with insulin-stimulated upregulation of IDE in cultured INS1E cells, as well as in rat and human islets. In conclusion, our study shows that IDE is expressed in pancreatic beta- and alpha-cells of both rodents and humans, having higher expression in alpha-cells. Furthermore, insulin stimulates IDE protein level in pancreatic beta-cells. These results may have implications in how DM2 patient’s treatment affects their beta-cell function.es
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dc.format.extent14es
dc.identifier.citationHistology and Histopathology, Vol.33, nº11, (2018)
dc.identifier.doiDOI: 10.14670/HH-11-997
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/120569
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectInsulin-degrading enzymees
dc.subjectType 2 diabeteses
dc.subjectInsulin treatmentes
dc.subjectOHAses
dc.subjectBeta-cellses
dc.subjectAlpha-cellses
dc.subjectRodent isletses
dc.subjectHuman isletses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleInsulin degrading enzyme is up-regulated in pancreatic β cells by insulin treatmentes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.33,nº11 (2018)