Publication: Expression of clusterin in Crohn's disease of the terminal ileum
Loading...
Date
2001
Authors
Gassler, N. ; Autschbachl, F. ; Heuschen, G. ; Witzgal, R. ; Otto, H.F. ; Obermüller, N.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Crohn's disease (CD) is a chronic
inflammatory intestinal disorder with disturbance and
injury of the intestinal mucosal barrier, in which various
proinflammatory molecules as well as molecules with
antiinflammatory activity and cytoprotective function
are found to be expressed. We investigated whether
clusterin, a multifunctional cytoprotective protein, is
upregulated in Crohn's disease, because augmented
expression of clusterin is seen in many organs following
various forms of tissue injury. Human actively and
inactively inflamed ileal tissues from CD patients as well
as normal intestinal specimens from control patients
(normal ileum) were investigated by Western blot
analysis, immunohistochemisty and in situ hybridization.
As compared with controls, a strongly enhanced
expression of clusterin was found in CD tissues,
correlating with disease activity. Immunohistochemistry
and in situ hybridization analysis revealed foci of crypts
almost completely lined by clusterin expressing
enterocytes in CD, a feature that was never seen in
controls. Such crypts appeared especially within the
morphologically intact mucosa apart from erosive or
ulcerative lesions. Besides epithelia, clusterin was also
expressed by inflammatory mononuclear cells. Enhanced
expression of clusterin by crypt epithelia might reflect a
cytoprotective function of the protein in order to prevent
further injury of the intestinal mucosal barrier in CD.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.