Publication:
Early effects of high-fat diet, extra-virgin olive oil and vitamin D in a sedentary rat model of non-alcoholic fatty liver disease

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Trovato-33-1201-1213-2018.pdf(14.78 MB)
Date
2018
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Authors
Trovato, Francesca Maria ; Castrogiovanni, Paola ; Szychlinska, Marta Anna ; Purrello, Francesco ; Musumeci, Giuseppe
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Publisher
Universidad de Murcia. Departamento de BiologĂ­a Celular e HistologĂ­a
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DOI
DOI: 10.14670/HH-18-008
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info:eu-repo/semantics/article
Description
Abstract
Background and Aim. Western high-fat diet is related to metabolic syndrome and non-alcoholic fatty liver disease (NAFLD). Decreased levels of Vitamin D (VitD) and IGF-1 and their mutual relationship were also reported. We aimed to evaluate whether different dietary profiles, containing or not VitD, may exert different effects on liver tissue. Methods. Twenty-eight male rats were fed for 10 weeks by different dietary regimens: R, regular diet; RDS and R-DR, regular diet with respectively VitD supplementation (DS) and restriction (DR); HFB-DS and HFB-DR (41% energy from fat), high fat (butter) diet; HFEVO-DS and HFEVO-DR (41% energy from fat), high fat (Extra-virgin olive oil-EVO) diet. Severity of NAFLD was assessed by NAFLD Activity Score. Collagen type I, IL-1beta, VitD-receptor, DKK-1 and IGF1 expressions were evaluated by immunohistochemistry. Results. All samples showed a NAS between 0 and 2 considered not diagnostic of steatohepatitis. Collagen I, although weakly expressed, was statistically greater in HFB-DS and HFB-DR groups. IL-1 was mostly expressed in rats fed with HFBs and HFEVOs and RDR, and almost absent in R and R-DS diets. IGF-1 and DKK-1 were reduced in HFBs and HFEVOs diets and in particular in DR groups. Conclusions. A short-term high-fat diet could damage liver tissue in terms of inflammation and collagen I deposition, setting the basis for the subsequent steatohepatitis, still not identifiable anatomopathologically. Vitamin D restriction increases inflammation and reduces the expression of IGF-1 in the liver, worsening the fat-induced changing. EVOO seems be protective against the collagen I production.
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Non-Alcoholic Fatty Liver Disease , Western Diet , Mediterranean Diet , Histology , Immunohistochemistry
Citation
Histology and Histopathology, Vol.33, nÂş11, (2018)
URI
http://hdl.handle.net/10201/120606
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Histology and histopathology Vol.33,nÂş11 (2018)
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