Publication: FOXO1 expression in villous trophoblast of
preeclampsia and fetal growth restriction placentas
Authors
Sheridan, Rachel ; Belludi, Chethan ; Khoury, Jane ; Stanek, Jerzy ; Handwerger, Stuart
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-30.213
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info:eu-repo/semantics/article
Description
Abstract
Oxidative stress and increased apoptosis are
implicated in the pathogenesis of many disorders of
pregnancy, including preeclampsia (PE) and fetal growth
restriction (FGR). Since the transcription factor FOXO1
(forkhead box protein O1) is implicated in the regulation
of a variety of cellular processes, including resistance to
oxidative stress, apoptosis and morphogenesis of the
placenta, we examined whether FOXO1 expression is
abnormal in placentas from patients with PE or FGR.
Paracentral sections from grossly unremarkable areas of
9 or 10 placentas each from early third trimester patients
(31.7±5.0 weeks) with mild PE, severe PE, FGR and a
gestational age-matched comparison group (GA
controls) were double immunostained for FOXO1 and
E-cadherin, the latter distinguishing villous
cytotrophoblast cells (CTB) from syncytiotrophoblast
(STB). The numbers of FOXO1-positive and FOXO1
negative STB and CTB nuclei were determined on ten
20x objective fields of each placenta section by three
observers who were blinded to the clinical outcome. The
results were evaluated by a generalized linear mixed
model. In mild PE, FOXO1-positive STB nuclei were
significantly decreased in number and FOXO1-negative
STB nuclei were increased as compared to GA controls.
However, the number of FOXO1-positive and FOXO1-
negative CTB nuclei were not significantly changes as
compared to GA controls. In severe PE and FGR, the
numbers of FOXO-positive and FOXO1-negative STB
and CTB were not statistically different from GA
controls. Since FOXO1 is critical for placental cellular
morphogenesis, abnormal FOXO1 expression may
contribute in part to the abnormal trophoblast
differentiation in mild PE. The differences in FOXO1
expression in mild and severe PE are consistent with
other studies suggesting that the two forms of PE are
different disease processes.
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Citation
Histology and Histopathology, Vol. 30, n.º 2 (2015)
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