Publication: Radial glia and cell debris removal during lesion-regeneration of the lizard medial cortex
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Date
1999
Authors
Nacher, J. ; Ramirez, C. ; Palop, J.J. ; Molowny, A. ; Luis de la Iglesia, J.A. ; López-García, Carlos
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Intraperitoneal injection of the neurotoxin 3-
acetylpyridine (3AP) induces a rapid degeneration of the
medial cerebral cortex (lizard fascia dentata) granular
layer and of its zinc enriched axonal projection (lizard
mossv fibres). After 6-8 weeks ~ost-lesionth e cell debris
have ieen rekoved and the grakular layer is repopulated
by neurons generated in the subjacent ependyma. Both
processes, neuron incorporation and debris removal,
seem to be crucial for successful regeneration.
Scavenging processes in the lesioned mammalian CNS
are usually carried out by microglia andlor astrocytes. In
the lizard cerebral cortex there are no free astrocytes and
the only glial fibrillary acid (GFAP) immunoreactive
cells are radial glia-ependymocytes, similar to
those present during mammalian CNS development.
Ependymocytes, in addition to their help in vertical
migrations of just generated immature neurons, built the
cortical glial scaffold, insulate the blood capillaries, form
the outer glial limiting membrane, thus playing an
essential role in the lizard cortical blood-brain barrier. In
this study, by means of GFAP-immunocytochemistry
and electron microscopy, we have shown that radial glial
cells participate actively in the removal/phagocytosis of
cellular debris generated in the lesion process: mainly
degenerated synapses, but interestingly, also some
neuronal somata. Cell debris taken up by ependymocyte
lateral processes seem to be progressively transported to
either distal (pial) or proximal (ventricular) poles of the
cell, where they result in lipofuscin accumulations. The
hypothetical subsequent exchange of debris from ependymoglia by microglia/macrophages and Kolmer
cells is discussed.
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