Publication: Histochemical study of lymphocystis disease in skin of gilthead seabream, Sparus aurata L.
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Date
1998
Authors
Sarasquete, C. ; Gonzalez de Canales, M.L. ; Arellano, J.M. ; Pérez-Prieto, S. ; García-Rosado, E. ; Borrego, J.J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
A battery of horseradish peroxidasecorijugated
lectiris (Con A, WGA arid DBA). as well as
coriventional histochemical techniques (PAS.
saponification, Alcian Blue pH 0.1 , 1. 2.5. chlorhydric
hydrolisis. sialidasc, Bromophenol blue, Tioglycollate
reduction and Ferric-ferricyanide-FeIlI) were used to
study the coritent and distribution of carbohydrates,
proteins and glycoconjugatc sugar residues on the skin
and on the lymphocystis-infected cells of gilthead
seabrcain, Spcrrus uurntcr. Variable ari-iounts of
glycoproteins containing sialic acid, N-acetyl-Dglucosainine,
N-acetyl-D-galactosarnine; mannose
andlor glucose residues were observed in the cuticle and
mucous cells of the c o r ~ o r a ls kin. tails and f ins . ~ ~
Germinative and epithelial cells of the epidermis
contained glycogen. proteins. carboxylated groups, as
well as glycoproteins with mannose andlor glucose and
N-acetyl-U-galactosamine residues. Hyaline capsule of
the mature lyniphocystis-infected cells was strongly
stained with PAS, Alcian Blue (pH 0.5 and 2.5) and
weakly positive with Alcian Blue (pH 1 ) . Con A reacted
with the granular cytoplasm, specially around hyaline
capsule. and with the basophilic intracytoplasinic
inclusions developed in mature lymphocystis-infected
cells of Spcrrrrs crlirntu skin. These sugar residues (niannose andlor glucose), as well as N-acetyl-Drrlucosamine
andlor sialic acid and N-acetvl-D- L
galactosamine were not detected in the hyaline capsule
of the lyr.iphocysti5 disease.
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