Publication: The absence of one or both nidogens does not alter basement membrane composition in adult murine kidney
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Date
2007
Authors
Gersdorff, N. ; Otto, S. ; Roediger, Matthias ; Kruegel, J. ; Miosge, Nicolai
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Nidogen-1 and nidogen-2 are major
components of all basement membranes and are
considered to function as link molecules between
laminin and collagen type IV networks. Surprisingly, the
knockout of one or both nidogens does not cause defects
in all tissues or in all basement membranes. In this study,
we have elucidated the appearance of the major
basement membrane components in adult murine kidney
lacking nidogen-1, nidogen-2, or both nidogens. To this
end, we localized laminin-111, perlecan, and collagen
type IV in knockout mice, heterozygous (+/-) or
homozygous (-/-) for the nidogen-1 gene, the nidogen-2
gene, or both nidogen genes with the help of light
microscopic immunostaining. We also performed
immunogold histochemistry to determine the occurrence
of these molecules in the murine kidney at the
ultrastructural level. The renal basement membranes of
single knockout mice contained a similar distribution of
laminin-111, perlecan, and collagen type IV compared to
heterozygous mice. In nidogen double-knockout
animals, the basement membrane underlying the tubular
epithelium was sometimes altered, giving a diffuse and
thickened pattern, or was totally absent. The normal or
thickened basement membrane of double-knockout mice
also showed a similar distribution of laminin-111,
perlecan, and collagen type IV. The results indicate that
the lack of nidogen-1, nidogen-2, or both nidogens,
plays no crucial role in the occurrence and localization
of laminin-111, collagen type IV, and perlecan in murine
tubular renal basement membranes
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