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FISH analysis for diagnostic evaluation of challenging melanocytic lesions

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dc.contributor.authorZimmermann, Anne-Katrines
dc.contributor.authorHirschmann, Astrides
dc.contributor.authorPfeiffer, David
dc.contributor.authorParedes, Bruno E.
dc.contributor.authorDiebold, Joachim
dc.date.accessioned2015-09-29T07:18:14Z
dc.date.available2015-09-29T07:18:14Z
dc.date.issued2010
dc.description.abstractThe differential diagnosis of malignant melanomas and atypical melanocytic nevi is still a diagnostic challenge. The currently accepted morphologic criteria show substantial interobserver variability, likewise immunohistochemical studies are often not able to discriminate these lesions reliably. Techniques that support diagnostic accuracy are of the greatest importance considering the growing incidence of malignant melanomas and their increase in younger patients. In this study we analyzed the feasibility of fluorescence in situ hybridization (FISH) analysis for the discrimination of malignant and benign melanocytic tumors. A panel of DNA probes was used to detect chromosomal aberrations of chromosomes 6 and 11. On a series of 5 clearly malignant and benign melanocytic tumors we confirmed the applicability of the test. Then we focused on examination of ambiguous melanocytic lesions, where atypical cells are often difficult to relocalize in the 4',6-Diamidino-2-phenylindol (DAPI)- fluorescence stain. FISH analyses were conducted on destained H&E-stained slides. By comparison of the DAPI-image with photos taken from the H&E stain, unambiguous assignment of the FISH results to the conspicuous groups of cells was possible. The results of FISH analysis were consistent with the conventional diagnosis in 11 of 14 small ambiguous lesions. Of the remaining 3 cases, 2 showed FISH-results close to the cut-off level. Comparison of FISH results on thin and thick sections revealed that the cut-off values have to be adopted for 2 µm destained sections. In conclusion, FISH analysis is a useful and applicable tool for assessment of even smallest melanocytic neoplasms, although there will remain unclear cases that cannot be solved even after additional FISH evaluation.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/46338
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectMelanocytic lesiones
dc.subjectDiagnosticses
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titleFISH analysis for diagnostic evaluation of challenging melanocytic lesionses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.25, nº9 (2010)