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Role of polymorphonuclear neutrophils (PMNs) and NK cells in the protection conferred by different vaccines against Chlamydophila abortus infection

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dc.contributor.authorOrtega, M.
dc.contributor.authorCaro, M. R.
dc.contributor.authorGallego, M. C.
dc.contributor.authorRío, L. del
dc.contributor.authorNicolás, L.
dc.contributor.authorCuello, F.
dc.contributor.authorSalinas, J.
dc.contributor.authorMartínez Cáceres, Carlos Manuel
dc.contributor.authorBuendía Marín, Antonio Julián
dc.contributor.departmentAnatomía y Anatomía Patológica Comparadas
dc.date.accessioned2025-01-21T09:17:52Z
dc.date.available2025-01-21T09:17:52Z
dc.date.issued2007-06
dc.description© 2006 Elsevier Ltd. This document is the Published version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2006.07.016es
dc.description.abstractOvine enzootic abortion (OEA) is caused by Chlamydophila abortus, an intracellular bacterium which acts by infecting the placenta, causing abortion in the last term of gestation. The main prevention strategy against OEA is the vaccination of flocks. An effective vaccine against C. abortus must induce a Th1-like specific immune response, which is characterized by the early production of IFN-γ and the activation of CD8+T cells. Moreover, vaccine effectiveness could be modulated by the functioning of the innate immunity. The purpose of this study was to ascertain how polymorphonuclear neutrophils (PMNs) and NK cells might influence vaccine-induced protection. The live attenuated 1B vaccine and two inactivated experimental vaccines, adjuvated with aluminium hydroxide (AH) or QS-21 (QS), were used in PMN-depleted or NK cell-depleted mice. For PMN depletion, RB6-8C5 monoclonal antibody, which recognizes GR1+receptors (Robben, P.M., LaRegina, M., Kuziel, W.A., Sibley, L.D. 2005. Recruitment of Gr-1+ monocytes is essential for control of acute toxoplasmosis. The Journal of Experimental Medicine 201, 1761–1769.) was used, while for NK cell-depletion the anti-asialo GM1 polyclonal antibody was used. The depletion of PMNs caused 100% mortality in non-vaccinated mice (NV) and 60% mortality in the AH-vaccinated mice by day 10 p.i., while both groups showed a significant increase in their bacterial burden in the liver by day 4 p.i. The depletion of NK cells caused mortality only in the NV group (50% by day 10 p.i.), although this group and the 1B vaccinated mice showed an increased bacterial burden in the liver at day 4 p.i. Our results suggest that the importance of PMNs in inactivated vaccines depends on the adjuvant chosen. The results also demonstrated that the importance of NK cells is greater in live vaccines than in inactivated vaccines.es
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dc.format.extent9es
dc.identifier.citationResearch in Veterinary Science, 2007, Vol. 82, Issue 3, pp. 314-322
dc.identifier.doihttps://doi.org/10.1016/j.rvsc.2006.07.016
dc.identifier.issnPrint: 0034-5288
dc.identifier.issnElectronic: 1532-2661
dc.identifier.urihttp://hdl.handle.net/10201/148890
dc.languageenges
dc.publisherElsevieres
dc.relationThis work was supported by a grant from MEC (AGL2004-06571). N. Ortega and C.M. Martínez were the recipients of predoctoral grants from the University of Murcia and The Spanish Ministry of Science and Technology, respectively.es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0034528806001640?via%3Dihubes
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectChlamydophila abortuses
dc.subjectVaccinees
dc.subjectAdjuvantes
dc.subjectInnate immune responsees
dc.subjectMouse modeles
dc.titleRole of polymorphonuclear neutrophils (PMNs) and NK cells in the protection conferred by different vaccines against Chlamydophila abortus infectiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicationa11bfbe4-fe50-4173-a96d-aa785375a6be
relation.isAuthorOfPublicationc084ce85-206c-418d-8cec-7f85a0f34dc0
relation.isAuthorOfPublication.latestForDiscoverya11bfbe4-fe50-4173-a96d-aa785375a6be
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