Publication: Different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle
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Date
1998
Authors
Radaelli, G. ; Domeneghini, C. ; Arrighi, S. ; Mascarello, F. ; Veggetti, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The presence of putative neuromodulators in
the nerve fibres was investigated in white skeletal
muscle of two teleost fish not taxonomically correlated
and showing different patterns of innervation (multiple
versus focal innervation). Cryostat sections of epaxial,
hypaxial and adductor mandibulae (AM) muscles of
Sparus aurata and Anguilla anguilla were stained
histochemically for reduced nicotinamide adenine
dinucleotide phosphate (NADPH)-diaphorase. Other
sections were used for indirect immunohistochemistry
(streptavidin-biotin and rhodamine immunofluorescence
methods), employing antibodies specific for putative
excitatory or inhibitory peptides, including CGRP,
substance P, met-enkephalin, bombesin, and VIP. In
addition, ultrastructural observations were performed in
order to describe the morphology of the motor endplates.
A strong immunoreactivity for CGRP and substance P
was found in many nerve terminals. Met-enkephalin,
bombesin and VIP immunoreactivities were less
frequently observed. No immunoreactivity was observed
to CCK, NPY or 5-HT. NADPH-diaphorase was
identified in nerve fibres of the AM complex only of A.
anguilla. Electron microscopy observations evidenced
more than one type of synaptic vesicle in motor
endplates. Some differences in putative neuromodulator
distributions were observed in the two species and
muscle complexes, which may be related to the different
taxonomical position as well as the different pattern of
innervation of white muscle fibres.
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