Publication: Regional variation in ontogeny
of class II antigens in enterocytes
of mouse small intestine
Authors
Sidhu, Nirmal K. ; Wrightk, Glenda M. ; Markham, R.J. Fred ; Singh, Amreek
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The ontogeny of major histocompatibility
class I1 antigens in small intestine enterocytes of
postnatal C3HlHe mice was investigated. Cryosections
of duodenal, jejunal, and ileal segments from 7-, 14-,
16-, 20-, 21-, 23-, 25-, 27-, 28-day-old and 7-week-old
mice were stained for the class I1 antigens with MRC
OX6 monoclonal antibodies by peroxidase-antiperoxidase
labelling. In adults, the duodenum exhibited
least expression of class I1 antigens that increased
progressively towards the ileum. The expression in the
villous epithelium was first seen in the duodenum and
jejunum 21 days after birth but the ileal enterocytes
did not exhibit any class I1 antigens. The earliest
appearance (21 days postnatal) of class 11 antigens in
the enterocytes coincides with the age of weaning
which suggests that immunologic stimulation by
ingested antigens after weaning may influence
expression of these antigens. At day 28 after birth, the
duodenum and jejunum expressed levels comparable
to those in the adults. The first expression of the
antigens seen in the ileum was at day 28 postpartum.
Crypt epithelium of the three regions of the small
intestine showed expression similar to that of
corresponding regional villous enterocytes. We
conclude that there is an age-dependent regional
variation in the expression of class I1 antigens in
enterocytes, and the expression increases with age.
The variation in expression of the class I1 antigens in
enterocytes of postnatal mice is attributed to the
developmental status of the tissue. The nature of
postnatal expression of the antigens is important since
an early appearance of these antigens may have
implications in autoimmunity.
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