Publication: Visualization of viral assembly in the infected cell
Authors
Risco, C. ; Carrascosa, J.L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The study of the virus life cycle in infected
cells is a methodological challenge due to the small size
and diversity of the viral components. Recent
developments on preservation of fine structure and
molecular localization have provided a group of
powerful methods with wide applications in cell biology
and virology. Among the different electron microscopy
(EM) techniques available to visualize viral assembly at
the intracellular level, we will focus on conventional
ultrathin sections, cryosections, and freeze-substitution.
For obtaining molecular information associated to
ultrastructure we have now a group of methods to detect
viral proteins (immunogold labeling), as well as the viral
genome, through the different techniques for detection of
nucleic acids (the enzyme-gold approach, in situ
hybridization, and elemental mapping). We will illustrate
the applications of these methods with examples of
viruses that exhibit different levels of structural
complexity. These new approaches help to detect and
identify viruses in clinical samples and to characterize
the virus life cycle and the cellular components
involved, to obtain data that could help for a therapeutic
intervention, and to characterize virus-like particles that
can be the basis of new and safe vaccines.
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