Publication: Endothelial cell activation mediated by cold ischemia-released mitochondria is partially inhibited by defibrotide and impacts on early allograft function following liver transplantation
Authors
Villalba-Lopez, Francisco ; Mateo, Sandra V. ; Vidal-Correoso, Daniel ; Jover-Aguilar, Marta ; Alconchel, Felipe ; Martínez-Alarcón, Laura ; López-López, Víctor ; Rios-Zambudio, Antonio ; Cascales, Pedro ; Pons Miñano, José Antonio ; Ramírez, Pablo ; Baroja-Mazo, Alberto ; García Bernal, David ; Pelegrín Vivancos, Pablo
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.biopha.2023.115529
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info:eu-repo/semantics/article
Description
© 2023 The Authors. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. This document is the Published version of a Published Work that appeared in final form in Biomedicine and Pharmacotherapy. To access the final edited and published work see https://doi.org/10.1016/j.biopha.2023.115529
Abstract
DAMPs (danger-associated molecular patterns) are self-molecules of the organism that appear after damage. The endothelium plays several roles in organ rejection, such as presenting alloantigens to T cells and contributing to the development of inflammation and thrombosis. This study aimed to assess whether DAMPs present in the organ preservation solution (OPS) after cold ischemic storage (CIS) contribute to exacerbating the endothelial
response to an inflammatory challenge and whether defibrotide treatment could counteract this effect. The activation of cultured human umbilical vein endothelial cells (HUVECs) was analyzed after challenging with endischemic OPS (eiOPS) obtained after CIS. Additionally, transwell assays were performed to study the ability of eiOPS to attract lymphocytes across the endothelium. The study revealed that eiOPS upregulated the expression of MCP-1 and IL-6 in HUVECs. Moreover, eiOPS increased the membrane expression of ICAM-1and HLA-DR, which facilitated leukocyte migration toward a chemokine gradient. Furthermore, eiOPS demonstrated its chemoattractant ability. This activation was mediated by free mitochondria. Defibrotide was found to partially inhibit the eiOPS-mediated activation. Moreover, the eiOPS-mediated activation of endothelial cells (ECs) correlated with early allograft dysfunction in liver transplant patients. Our finding provide support for the hypothesis that mitochondria released during cold ischemia could trigger EC activation, leading to complications in graft outcomes. Therefore, the analysis and quantification of free mitochondria in the eiOPS samples obtained after CIS could provide a predictive value for monitoring the progression of transplantation. Moreover, defibrotide emerges as a promising therapeutic agent to mitigate the damage induced by ischemia in donated organs.
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Citation
Biomedicine & Pharmacotherapy 167 (2023) 115529
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