Publication: Expression and potential role of phospholipase D1 in cryoinjured cerebral cortex of rats
Authors
Kim, M.D. ; Min, D.S. ; Sim, K.B. ; Cho, H.J. ; Shin, T.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The expression and potential role of
phospholipase D1 (PLD1) were studied in the cerebral
cortex of rats after freeze injury. Histopathologically,
cryoinjury, by exposing cerebral cortex to a prechilled
rod for 1 minute, produced consistent pathological
lesions, specifically neuronal death, infiltration of
macrophages into the center of the cryoinjury, and
reactive astrogliosis at the periphery, which caused the
lesion site to become encased. Western blot analysis
showed that PLD1 expression in the ipsilateral cerebral
cortex increased significantly during days 1 to 3 after
cryoinjury and declined slightly at post-injury day 7.
PLD1 immunoreactivity was very low in the brains of
sham-operated control adults. After cryoinjury, there was
substantial PLD1 immunostaining of numerous
inflammatory cells in the ipsilateral cortex, which were
identical to ED1-positive macrophages. In addition,
PLD1 immunoreactivity was increased in some neurons
and astrocytes at the periphery of the cryoinjury at postinjury
days 3 and 7. These findings suggest that
cryoinjury by means of prechilled rods induced
consistent histopathological changes in the cerebral
cortex. In addition, expression of a cell activation signal,
PLD1, was upregulated in macrophages and astrocytes
in the ipsilateral cerebral cortex after cryoinjury
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