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Structural and functional integrity of endocrine pancreas post administration of Karwinskia humboldtiana fruit to Wistar rats: a possible therapeutic application for cancer of exocrine origin

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dc.contributor.authorSegoviano Ramirez, Juan Carlos
dc.contributor.authorEsparza Rodriguez, Nallely
dc.contributor.authorCarcano Diaz, Katya
dc.contributor.authorDiaz Perez, Rosa Nelly
dc.contributor.authorPalma Nicolas, Jose Prisco
dc.contributor.authorHernandez Bello, Romel
dc.contributor.authorGarcía Juárez, Jaime
dc.date.accessioned2023-09-11T09:08:16Z
dc.date.available2023-09-11T09:08:16Z
dc.date.issued2023
dc.description.abstractAims. Pancreatic adenocarcinoma represents a therapeutic challenge due to the high toxicity of antineoplastic treatments and secondary effects of pancreatectomy. T-514, a toxin isolated from Karwinskia humboldtiana (Kh) has shown antineoplastic activity on cell lines. In acute intoxication with Kh, we reported apoptosis on the exocrine portion of pancreas. One of the mechanisms of antineoplastic agents is the induction of apoptosis, therefore our main objective was to evidence structural and functional integrity of the islets of Langerhans after the administration of Kh fruit in Wistar rats. Methods. TUNEL assay and immunolabelling against activated caspase-3 were used to detect apoptosis. Also, immunohistochemical tests were performed to search for glucagon and insulin. Serum amylase enzyme activity was also quantified as a molecular marker of pancreatic damage. Results. Evidence of toxicity on the exocrine portion, by positivity in the TUNEL assay and activated caspase-3, was found. On the contrary, the endocrine portion remained structurally and functionally intact, without apoptosis, and presenting positivity in the identification of glucagon and insulin. Conclusions. These results demonstrated that Kh fruit induces selective toxicity on the exocrine portion and establish a precedent to evaluate T-514 as a potential treatment against pancreatic adenocarcinoma without affecting the islets of Langerhans.es
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dc.format.extent9es
dc.identifier.citationHistology and Histopathology Vol. 38, nº9 (2023)
dc.identifier.doihttps://doi.org/10.14670/HH-18-603
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/133807
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAdenocarcinomaes
dc.subjectKarwinskia humboldianaes
dc.subjectApoptosises
dc.subjectIslets of Langerhanses
dc.subjectDiabeteses
dc.subjectSelective toxicityes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.1 - Patología del sistema circulatorio, de los vasos sanguíneos. Transtornos cardiovasculareses
dc.titleStructural and functional integrity of endocrine pancreas post administration of Karwinskia humboldtiana fruit to Wistar rats: a possible therapeutic application for cancer of exocrine origines
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.38, nº9 (2023)