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Nrf1 is time-dependently expressed and distributed in the distinct cell types after trauma to skeletal muscles in rats

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dc.contributor.authorZhang, Shu-Tao
dc.contributor.authorZhao, Rui
dc.contributor.authorMa, Wen-Xiang
dc.contributor.authorFan, Yan-Yan
dc.contributor.authorGuan, Wen-Zheng
dc.contributor.authorWang, Jiao
dc.contributor.authorRen, Peng
dc.contributor.authorZhong, Kun
dc.contributor.authorYu, Tian-Shui
dc.contributor.authorPi, Jing-Bo
dc.contributor.authorGuan, Da-Wei
dc.date.accessioned2018-05-16T11:04:56Z
dc.date.available2018-05-16T11:04:56Z
dc.date.issued2013
dc.description.abstractOur goal was to elucidate the dynamic expression and distribution of the nuclear factor erythroid-derived factor 2-related factor 1 (Nrf1) by immunohistochemistry, Western blotting, and real-time PCR during wound healing of contused skeletal muscle in rats. An animal model of skeletal muscle contusion was established in 40 Sprague-Dawley male healthy rats. Samples were taken at 6 h, 12 h, 1 day, 3 days, 5 days, 7 days, 10 days, and 14 days post-injury, respectively (5 rats in each posttraumatic interval). 5 rats were employed as control. A weak immunoreactivity of Nrf1 was observed in the sarcoplasm and nuclei of normal myofibers in control rats. Prominent immunostaining for Nrf1 was seen in a large number of polymorphonulcear cells, round-shaped mononuclear cells and spindleshaped fibroblastic cells, and regenerated multinucleated myotubes in the injured tissue. Subsequently, neutrophils, macrophages and myofibroblasts were identified as expressing Nrf1 by double immunofluorescent procedures. By real-time PCR analysis, Nrf1 expression was up-regulated and peaked at inflammatory phase. The expression tendency was also confirmed by Western blot. In conclusion, Nrf1 is time-dependently expressed in certain cell types, such as neutrophils, macrophages, myofibroblasts and regenerated multinucleated myotubes, suggesting that Nrf1 may modulate oxidative stress response and regeneration after trauma to skeletal muscles.es
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.citationHistology and Histopathology, vol. 28, nº 6, (2013)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/58442
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectSkeletal musclees
dc.subjectTraumaes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleNrf1 is time-dependently expressed and distributed in the distinct cell types after trauma to skeletal muscles in ratses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.28, nº 6 (2013)