Publication: Immunohistochemistry of connexin43 and
zonula occludens-1 in the myocardium as
markers of early ischemia in autopsy material
Authors
Kawamoto, Osamu ; Michiue, Tomomi ; Ishikawa, Takaki ; Maeda, Hitoshi
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Immunohistochemistry of the terminal
complement complex (C5b-9) and fibronectin (FN) is
useful to detect myocardial ischemia preceding necrosis
in the postmortem diagnosis of sudden cardiac death.
The present immunohistochemical study examined
connexin43 (Cx43) and zonula occludens-1 (ZO-1) as
markers of early myocardial ischemia in addition to the
above-mentioned markers, using forensic autopsy cases
of acute deaths due to myocardial infarction (MI, n=15)
and acute ischemic heart disease (AIHD) without
apparent myocardial necrosis (n=8), compared with
those of acute mechanical asphyxiation (As, n=24) and
drowning (D, n=10) as controls. Immunopositivities of
each marker in the myocardium were semi-quantitatively
graded by scoring. ZO-1, C5b-9 and FN were detected in
the myocardial cytoplasm, whereas Cx43 and nonphosphorylated
(np) Cx43 showed varied localizations at
the intercalated disc, in the cytoplasm and along the
lateral cell border. ZO-1 and FN showed a tendency to
be detected more intensely in MI and IHD than in As
and D. C5b-9 showed specific staining at the site of
ischemia in MI (n=10/15) and AIHD (n=6/8), while the
distribution of npCx43 was different in most cases of MI
(n=14/15) and AIHD (n=5/8), compared with As and D;
npCx43 positivity score was higher in the cytoplasm
than at the intercalated disc, indicating redistribution due
to myocardial ischemia. Such findings were detected in a
few cases of As (n=3/24). These findings suggest that
the combination of npCx43 and C5b-9 immunohistochemistry
is useful for detecting early lesions of
myocardial ischemia in sudden cardiac death.
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Citation
Histology and Histopathology, vol. 29, nº 6, (2014)
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