Publication: Aberrant nuclear beta-catenin expression in the spindle or corded cells in so-called corded and hyalinized endometrioid carcinomas.
Another critical role of the unique morphological feature
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Date
2009
Authors
Wani, Yoji ; Saegusa, Makoto ; Notohara, Kenji
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Corded and hyalinized endometrioid
carcinoma (CHEC), showing spindle and/or corded
(SPICO) cells often in the background of hyalinized
stroma, is a rare variant of uterine endometrioid
carcinomas. The aim of our study was to explore the
status of cell-adhesion molecules (beta-catenin, Ecadherin) in CHECs and to survey whether
immunostains for beta-catenin and p53 can help to
distinguish CHECs from their morphological mimics:
malignant mixed mullerian tumors (MMMTs) and
uterine tumors resembling ovarian sex-cord tumors
(UTROSCTs). Immunohistochemistry was performed
and scored for each element as follows: 0: negative, 1+:
<10% positive cells, 2+: 10-30%, 3+: >30%. The SPICO
patterns were classified as spindle/fusiform; 3, corded; 1,
and both; 2. SPICO components consisted of <10%: 4,
10-30%: 1, >30%: 1. Five contained squamous
components. In SPICO elements of all CHECs, nuclear
beta-catenin expression (score: 1+; 1, 2+; 2, 3+; 3) and
complete loss of membranous expression of E-cadherin
was observed. In contrast, comparable components
(sarcomatous ones for eight MMMTs or sex-cord-like
ones for six UTROSCTs) showed no nuclear positivity
for beta-catenin. p53 expression was observed in SPICO
(64.7%), sarcomatous (87.5%), and sex-cord-like (50%)
components, and sarcomatous areas of most MMMTs
notably showed diffuse and intense staining. Sequence
analysis of PCR amplification products of exon 3 of the
beta-catenin gene revealed mutation in all cases, except
two lacking SPICO components represented on
microdissected areas. Our results suggest that alterations
in beta-catenin/E-cadherin complex play a critical role in
SPICO features. Immunostain for beta-catenin and p53
is a promising approach for distinguishing CHECs from MMMTs and UTROSCTs
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