Publication: Defect of the cerebellar vermis induced by prenatal y-ray irradiation in radiosensitive BALB/c mice
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Date
2008
Authors
Saito, Aya ; Yamauchi, Hirofumi ; Ishida, Yuka ; Ohmachi, Yasushi ; Nakayama, Hiroyuki
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The developing fetal brain is one of the most
susceptible organs to irradiation insult. Prenatal
irradiation-induced abnormalities in the cerebrum have
been well examined in mouse fetuses. However, little
information on abnormalities in the cerebellum caused
by irradiation is available. Moreover, few reports have
examined the chronological changes of the brain from
the prenatal to the postnatal period. To analyze the
chronological changes induced by irradiation, we
exposed pregnant mice to g-ray irradiation on embryonic
day 13.5 (E13.5) and investigated the histopathology of
the cerebellum at several time points from E14.5 to
postnatal day 28. BALB/cA mice were used, which is a
radiosensitive strain, and C57BL/6J, which is a
radioresistant strain. The irradiated BALB/c showed a
remarkable vermis deficit after birth, and histological
analysis demonstrated that there were severe losses of
the external germinal layer (EGL) and Purkinke cell
layer. TUNEL analysis shoed that apoptosis was strongly
induce in the cerebellar anlage of the irradiated BALB/c
compared to the C57BL/6J at E14.5. Immunohistochemical
analysis revealed a significat decrease of
phospho-histone H3 positive EGL cells in the irradiated
BALB/c at E18.5 and E0, indicating that irradiation
causes a decrease in the number of mitotic cells. The
results suggest that the strong induction of apoptosis in radiosensitive BALB/c led to a decrease of proliferation
activity in the cerebellar anlage during embryonic
development, and consequently, severe cerebellar
abnormality was evoked.
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