Publication: Post-genomic applications of tissue microarrays: basic research, prognostic
oncology, clinical genomics and drug discovery
Authors
Mobasheri, A. ; Airley, R. ; Foster, C.S. ; Schulze-Tanzil, G. ; Shakibaei, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Tissue microarrays (TMAs) are an ordered
array of tissue cores on a glass slide. They permit
immunohistochemical analysis of numerous tissue
sections under identical experimental conditions. The
arrays can contain samples of every organ in the human
body, or a wide variety of common tumors and obscure
clinical cases alongside normal controls. The arrays can
also contain pellets of cultured tumor cell lines. These
arrays may be used like any histological section for
immunohistochemistry and in situ hybridization to detect
protein and gene expression. This new technology will
allow investigators to analyze numerous biomarkers over
essentially identical samples, develop novel prognostic
markers and validate potential drug targets. The ability
to combine TMA technology with DNA microarrays and
proteomics makes it a very attractive tool for analysis of
gene expression in clinically stratified tumor specimens
and relate expression of each particular protein with
clinical outcome. Public domain software allows
researchers to examine digital images of individual
histological specimens from TMAs, evaluate and score
them and store the quantitative data in a relational
database. TMA technology may be specifically applied
to the profiling of proteins of interest in other
pathophysiological conditions such as congestive heart
failure, renal disease, hypertension, diabetes, cystic fibrosis and neurodegenerative disorders. This review is
intended to summarize the strengths and weaknesses of
TMA technology which will have an increasingly
important role in the laboratories of the post-genomic
era.
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