Publication: Spatial-temporal protein expression of inhibitor
of differentiation-1 (Id1) during fetal embryogenesis
and in different mouse and human cancer types
Authors
Redrado, Miriam ; Bodegas, Elena ; Villaro, Ana Cristina ; Nguewa, Paul A. ; Lopez, Ines ; Gil-Bazo, Ignacio ; Calvo, Alfonso
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Inhibitor of differentiation-1 (Id1) plays a
role in cell proliferation, acquisition of epithelial to
mesenchymal transition (EMT) features and
angiogenesis. Id1 was shown to be expressed in some
tumor types, mainly in advanced dedifferentiated stages.
However, recent studies using a validated and highly
specific monoclonal antibody against Id1 have
challenged many of the results obtained by
immunohistochemistry. The goal of our work was to
perform a thorough analysis of Id1 expression in mouse
embryos and adult tissues, as well as healthy and
malignant mouse and human samples using this
validated antibody (Perk et al., 2006). Our results show
that Id1 was highly expressed in the oropharyngeal
cavity, lung, cartilage and skin of E14 and E15 mouse
embryos, but expression was progressively reduced in
more developed embryos. Immunostaining only
remained in epithelial cells of the gut and uterus of adult
mice. Mammary MMTV-Myc and MMTV-Myc/VEGF
transgenic mouse tumors, and squamous cell carcinomas
of the lung induced by N-nitroso-tris-chloroethylurea
(NTCU) were highly positive for Id1, unlike their
respective healthy counterparts. Id1 immunostaining in a
human tissue microarray (TMA) revealed strong
expression in cancers of the oral cavity, bladder and
cervix. Some tumor specimens of esophagus, thyroid
and breast were also strongly positive. Our results
suggest that Id1 is an oncofetal protein highly expressed
in particular tumor types that should be reanalyzed in future studies using large cohorts of patients to reassess
its diagnostic/prognostic value. Moreover, MMTV-Mycand
NTCU-induced tumors could serve as appropriate
mouse models to study Id1 functions in breast and lung
cancer, respectively.
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Citation
Histology and Histopathology, vol. 28, nº 8 (2013)
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