Publication:
Hyperin up-regulates miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells

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Date
2023
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Authors
Qian, Dongchen ; Chen, Yueyue ; Qiu, Xusheng ; Zhu, Baohua ; Zhang, Lin ; Yan, Yifeng ; Chen, Yixin
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-579
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info:eu-repo/semantics/article
Description
Abstract
Objective. To investigate the effects of Hyperin (Hyp) on osteogenic differentiation of MC3T3E1 cells. Methods. Differentially expressed miRNA was screened by miRNA Microarray. miR-7031-5P overexpression and knockdown MC3T3-E1 cell models were constructed by transfecting miR-7031-5P mimics and inhibitor. Alizarin red staining (ARS) assay was used to observe the formation of mineralized nodules in MC3T3-E1 cells. ALP activity was detected by using ALP detection kit. Western blot assay was used to examine the changes in osteogenic differentiation-related proteins. The relationship between miR-7031-5P and Wnt7a was revealed by dual luciferase report experiments. Results. We found that miR-7031-5P was upregulated in MC3T3-E1 cells after Hyp treatment. The results indicated that compared with the untreated group, Hyp promoted the formation of mineralized nodules and the alkaline phosphatase (ALP) activity of MC3T3-E1 cells via overexpressing miR-7031-5P. Besides, elevated miR-7031-5P increased OPN, COL1A1, and Runx2 mRNA expression. More importantly, Wnt7a was identified as the downstream target gene of miR-70315P promoting osteogenic differentiation of MC3T3-E1 cells. Conclusions. Hyp up-regulated miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells by targeting Wnt7a
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Hyp , MC3T3-E1 , Wnt7a , Osteogenic differentiation , miR-7031-5P
Citation
Histology and Histopathology Vol. 38, nº10 (2023)
URI
http://hdl.handle.net/10201/134425
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Histology and histopathology Vol.38,nº10 (2023)
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