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Inflammatory cytokine and chemokine expression in sympathetic ophthalmia: a pilot study

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dc.contributor.authorFurusato, Emiko
dc.contributor.authorShen, DeFen
dc.contributor.authorCao, Xiaoguang
dc.contributor.authorFurusato, Bungo
dc.contributor.authorNussenblat, Robert B.
dc.contributor.authorRushing, Elisabeth J.
dc.contributor.authorChan, Chi-Chao
dc.date.accessioned2016-04-27T17:07:35Z
dc.date.available2016-04-27T17:07:35Z
dc.date.issued2011
dc.description.abstractSympathetic ophthalmia is a bilateral uveitis that develops after penetrating injury to one eye. This study aimed to identify the inflammatory cellular sub-phenotypes and expression of pertinent inflammatory cytokines/chemokines in sympathetic ophthalmia (SO). Dalen-Fuchs nodules (DFN), granulomas, and non-granulomatous foci of inflammation were micro-dissected from 15 cases. RNA was extracted, and quantitative PCR was performed to measure IL-17, IL-18, IL-23, IFN-γ, CCL19, CXCL11, CCL17, and CCL22 transcripts. Immunohistochemical methods were used to characterize CD3, CD4, CD8, CD20, CD68, and CD163 expression. Non-granulomatous lymphocytes were predominantly CD3-positive and expressed more IFN-γ than cells within granulomas, consistent with Th1 cells. In contrast, granulomas and DFN contained mainly CD68+, CD163+/- and expressed more IL-17, IL-18, IL-23, CCL19, and CXCL11 than non-granulomatous cells. Our data indicate for the first time that M1 macrophages are the predominant inflammatory cells within granulomas and DFN of SO. We further observed high levels of IL-17 within granulomas and the presence of Th1 and M1 cells.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/49385
dc.languageenges
dc.publisherF. Hernández y J.F. Madrid. Murcia: Universidad de Murcia, Departamento de Biología Celular e Histología.es
dc.relation.ispartofhistology and histopathology, vol. 26, nº9 (2011)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectChemokinees
dc.subjectCytokineses
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titleInflammatory cytokine and chemokine expression in sympathetic ophthalmia: a pilot studyes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.26, nº9 (2011)