Publication: Use of time-resolved immunofluorometric assay for determination of canine C-reactive protein concentrations in whole blood
Authors
Parra Muñoz, María Dolores ; Tuomola, Mika ; Cabezas-Herrera, Juan ; Cerón Madrigal, José Joaquín
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Publisher
American Veterinary Medical Association
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DOI
https://doi.org/10.2460/ajvr.2005.66.62
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info:eu-repo/semantics/article
Description
© 2005, American Veterinary Medical Association. This manuscript version is made available under the CC-BY-NC 4.0 license http://creativecommons.org/licenses/by-nc4.0/. This document is the Published version of a Published Work that appeared in final form in American Journal of Veterinary Research. To access the final edited and published work see https://doi.org/10.2460/ajvr.2005.66.62
Abstract
Objective: To develop and validate a time-resolved immunofluorometric assay (TR-IFMA) for measurement of C-reactive protein (CRP) in canine whole blood. Animals: 12 healthy dogs and 35 dogs with inflammatory processes. Procedure: CRP was isolated from acute-phase serum by affinity chromatography and used as a standard for calibration. Analytic and functional limit of detection and intra-assay and interassay precision were calculated. Accuracy was evaluated by recovery assays and by comparison with results of a commercial ELISA. Correlation between CRP concentrations in whole blood and corresponding plasma fractions was tested by use of TR-IFMA. Stability of blood samples at 4 degrees C was assessed during a 1-month period, and effects of anticoagulants were evaluated. Measurements of CRP in blood samples from 12 healthy dogs were compared with those of 35 dogs with inflammatory diseases. Results: Analytic and functional limits of detection were 0.53 and 3.26 microg/mL, respectively. Intra-assay and interassay coefficients of variation varied between 2.1% to 8.9% and 8.0% to 12.3%, respectively. Mean recoveries of added CRP were 104% and 114%. Measurements of CRP by use of TR-IFMA and ELISA were highly correlated (R2 = 0.97). Measurements of CRP in whole blood and in corresponding plasma fractions by use of TR-IFMA were also highly correlated (R2 = 0.97). Neither storage nor use of anticoagulants disturbed measurement of CRP concentrations in whole blood. Concentrations of CRP in whole blood of dogs with inflammation were significantly higher than in healthy dogs. Conclusions and clinical relevance: Determination of CRP concentrations in whole blood may provide a diagnostic test for inflammation in dogs.
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Citation
American Journal of Veterinary Research, 01 Jan 2005, 66(1):62-66
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