Publication: Circadian rhythms of clock gene expression in Nile tilapia (Oreochromis niloticus) central and peripheral tissues : Influence of different lighting and feeding conditions
Authors
Costa, Leandro ; Serrano, Ignacio ; Sánchez Vázquez, Francisco Javier ; López Olmeda, José Fernando
item.page.secondaryauthor
item.page.director
Publisher
Springer
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1007/s00360-016-0989-x
item.page.type
info:eu-repo/semantics/article
Description
©<2016>. This manuscript version is made available under the CC-BY license http://creativecommons.org/licenses/ccby/4.0/
This document is the Acepted, version of a Published Work that appeared in final form in [Journal of Comparative Physiology B].To access the final edited and published work see [https://doi.org/10.1007/s00360-016-0989-x]
Abstract
The present research aimed to investigate the existence of clock gene
expression rhythms in tilapia, their endogenous origin, and how light
and feeding cycles synchronize these rhythms. In the first experiment,
two groups of fish were kept under an LD cycle and fed at two different
time points: in the middle of the light (ML) or in the middle of the dark
(MD) phase. In the second experiment, fish fed at ML was fasted and
kept under constant lighting (LL) conditions for 1 day. In both
experiments, the samples from central ( optic tectum and hypothalamus)
and peripheral (liver) tissues were collected every 3 h throughout a 24 h
cycle. The express10n levels of clock genes bmall a, clockl, per 1 b,
cry2a, and cry5 were analyzed by quantitative PCR. All the clock genes
analyzed in brain regions showed daily rhythms: clockl, bmall a, and
cry2a showed the acrophase approximately at the end of the light phase
(ZT 8:43-11:22 h), whereas perlb and cry5 did so between the end of
the dark phase and the beginning of the light phase, respectively (ZT
21: 16-4:00 h). These rhythms persisted under constant conditions. No
effect of the feeding time was observed in the brain. In the liver,
however, the rhythms of clockl and cry5 were influenced by feeding,
anda shift was observed in the MD fish group (ZT 3:58 h for clockl and
11 :20 h for cry5). This study provides the first insights into the
molecular clock of tilapia, a very important fish species for aquaculture.
It also reveals the endogenous origin of clock gene rhythms and the
ability of feeding time to shift the phase in sorne clock genes in the
peripheral, but not the central, oscillator.
publication.page.subject
Citation
Journal of Comparative Physiology B 186, 775–785 (2016)
item.page.embargo
Collections
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/