Publication: Localization of integrin αvß3 and vascular endothelial growth factor receptor-2
(KDR/Flk-1) in cutaneous and oral melanomas of dog
Authors
Rawlings, N.G. ; Simko, E. ; Bebchuk, T. ; Caldwell, S. ; Singh, B.
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Melanomas are common neoplasms of dogs
and arise from pigment-producing cells called
melanocytes or melanoblasts. Melanomas of skin are
often easily cured by surgical excision, but those of oral
mucosa are aggressive, metastasize to the regional
lymph nodes and lungs, and respond poorly to
conventional therapy. Tumor growth is sustained by
proliferation of microvessels via a process called
angiogenesis. Integrin
α
vß3 is expressed in proliferating
but not in quiescent microvessels suggesting a role in
angiogenesis. Vascular endothelial growth factor
(VEGF) manifests its mitogenic and angiogenic effects
mainly via VEGF receptor-2 (VEGFR-2/Flk-1). We
conducted this immunocytochemical study to investigate
the expression of integrin
α
vß3 and VEGFR-2 in
archival and fresh samples from cases of canine
melanomas. Results show that integrin
α
vß3 was
expressed in 72% and 88% of cutaneous and oral
melanomas, respectively, and the expression was
restricted to and immediately around the melanocytes
and endothelial cells. VEGFR-2 staining of selected
cases of melanoma revealed that its expression
overlapped with the
α
v
β
3 integrin. Dual immuno-gold
electron microscopy confirmed co-localization of
integrin
α
vß3 and VEGFR-2 in melanocytes and
endothelial cells. These data demonstrate expression and
co-localization of integrin
α
vß3 and VEGFR-2 in
cutaneous and oral melanomas of dogs.
Citation
Histology and histopathology, Vol. 18, nº 3 (2003)
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