Publication: Preservation of human skin structure and function in organ culture
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Date
1998
Authors
Varani, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Human keratinocytes can be maintained in
monolayer culture under serum-free conditions for an
extended period of time. Under low ca2+ conditions
(e.g., 0.05-0.15 mM), an undifferentiated state is
maintained and the cells proliferate optimally. When the
ca2+ concentration is raised to approximately 1.0 mM,
differentiation occurs and growth slows. Human dermal
fibroblasts can also be maintained in monolayer culture
under serum-free conditions, but in contrast to
keratinocytes, a physiological level of extracellular ca2+
(above approximately 1.0 mM) is required. A variety of
growth factors stimulate roliferation of both cell types
but do not replace the CaP+ requirement of the fibroblast
population. All-trans retinoic acid also promotes
proliferation of both cell types and, most interestingly,
replaces the requirement for a physiological level of
ca2+ in the fibroblast cultures. Human skin can be
maintained in organ culture for an extended period of
time under serum-free conditions. Conditions optimized
for fibroblast proliferation (either physiological ca2+ or
all-trans retinoic acid) are required. In the presence of
culture conditions optimized for the epithelial cell
component, both the epidermis and dermis rapidly lyse.
These data suggest that the fibroblast is the critical
component in maintaining homeostasis of skin, and that
maintenance of the epidermis as well as the dermis
depends on the viability and functioning of these cells.
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