Publication: Differential location and structural specificities of sialic acid-ß-D-Gal sequences belonging to sialoderivatives of
rabbit oviduct under hormonal treatment
Authors
Gabrielli, M.G. ; Bondi, A.M. ; Materazzi, G. ; Menghi, Giovanna
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Sialoderivatives expressed in the rabbit
oviduct under hormonal treatment have been
investigated in situ by lectin histochemistry with a view
to specifying further regional and temporal
specializations which enable ampulla and isthmus to
play distinct roles in the reproductive events.
Application of MAL II and SNA lectins allowed
sialoglycoconjugates containing Sia(a2,3)Gal and
Sia(a2,6)Gal groups to be discriminated. Sialic acid
residues linked to Gal(ß1,3)-D-GalNAc sequences were
identified using PNA combined with sialidase digestion.
Information on structural features of sialic acids were
acquired by deacetylation and differential oxidation
pretreatments. In both oviductal portions, Sia(a2,6)
groups were restricted to the luminal surface of the
lining epithelium while Sia(a2,3) groups were
specifically located in the secretory, non-ciliated cells. In
the ampullary epithelium, non-acetylated sialic acids
a2,3-linked to Gal(ß1,3)-D-GalNAc sequences were
largely present. Only at ovulation time were sialic acid
residues containing acetyl substituents on C4 also found.
A great variety of sialic acids were found in the isthmic
epithelium which showed the highest expression of
acetylated forms at the first hours after the hormonal
treatment. The heterogeneity of sialoderivatives differently expressed in the ampulla and isthmus as well
as their distinct cycle-dependent modulation suggest that
sialylated components may contribute to the molecular
and functional specificities within the oviductal
epithelium.
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