Publication: Apoptosis resistance in
pigmented villonodular synovitis
Authors
Berger, I. ; Aulmann, S. ; Ehemann, V. ; Helmchen, B. ; Weckauf, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Objective: Pigmented villonodular synovitis
(PVNS) is a proliferative lesion originating from
synovial tissue with a locally aggressive behaviour. We
analysed the pathogenetic role of apoptosis resistance for
sustained cell proliferation in PVNS. Methods: The
expression of bcl-2, p53 and Ki-67 was examined in 80
cases of PVNS using immunohistochemistry. In 43 of
these cases, DNA content and distribution of cell-cycle
phases were investigated by flow cytometry.
Additionally, 10 cases of PVNS were analysed by multiparametric
flow cytometry for expression of p53,
caspase3, and bcl-2 and by TUNEL to detect DNA
fragmentation. Results: No apoptotic cell fractions were
detected in any investigated cases. Expression of bcl-2
was found in 84% of cases (up to 6.5% of cells) and was
significantly associated with DNA-fragmentation
observed by TUNEL (p=0.037). Orthologous p53
expression was observed in 37% of cases. The level of
p53 expression correlated with the proliferative activity
and the expression of both caspase3 (p=0.017) and bcl-2
(p=0.0013). (No statistically significant correlations
between expression of bcl-2, p53, caspase3, DNA
fragmentation or proliferative index and age, sex of
patients, disease recurrence, growth pattern or size of
lesion were found). Conclusion: Apoptosis resistance is
a critical event in the progression of PVNS and may
contribute to the survival of the proliferating synovial
cells in PVNS and to the permanent slow progression of
these lesions.
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