Publication: Altered distribution of extracellular matrix proteins
in the periodontal ligament of periostin-deficient mice
Date
2014
Authors
Tabata, Chihiro ; Hongo, Hiromi ; Sasaki, Muneteru ; Hasegawa, Tomoka ; Luiz de Freitas, Paulo Henrique ; Yamada, Tamaki ; Yamamoto, Tomomaya ; Suzuki, Reiko ; Yamamoto, Tsuneyuki ; Oda, Kimimitsu ; Li, Minqi ; Kudo, Akira ; Iida, Junichiro ; Amizuka, Norio
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Verifying whether periostin affects the
distribution of type I collagen, fibronectin and tenascin
C in the periodontal ligament (PDL) is important to
contribute to a more thorough understanding of that
protein’s functions. In this study, we have histologically
examined incisor PDL of mandibles in 20 week-old male
wild-type and periostin-deficient (periostin-/-) mice, by
means of type I collagen, fibronectin, tenascin C,
proliferating cell nuclear antigen, matrix metalloproteinase
(MMP)-1 and F4/80-positive monocyte/
macrophage immunostaining, transmission electron
microscopy and quantitative analysis of cell
proliferation. Wild-type PDL featured well-arranged
layers of collagen bundles intertwined with PDL cells,
whose longitudinal axis ran parallel to the collagen
fibers. However, cells in the periostin-/- PDL were
irregularly distributed among collagen fibrils, which
were also haphazardly arranged. Type I collagen and
fibronectin reactivity was seen throughout the wild-type
PDL, while in the periostin-/- PDL, only focal, uneven
staining for these proteins could be seen. Similarly,
tenascin C staining was evenly distributed in the wildtype
PDL, but hardly seen in the periostin-/- PDL. MMP1
immunoreactivity was uniformly distributed in the
wild-type PDL, but only dotted staining could be
discerned in the periostin-/- PDL. F4/80-positive
monocyte/macrophages were found midway between
tooth- and bone-related regions in the wild-type PDL, a
pattern that could not be observed in the periostin-/-
PDL. In summary, periostin deficiency may not only
cause PDL collagen fibril disorganization, but could also
affect the distribution of other major extracellular matrix
proteins such as fibronectin and tenascin C.
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Citation
Histology and Histopathology, vol. 29, nº 6, (2014)
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