Preventing legionellosis outbreaks by a quick detection of airborne Legionella pneumophila

Sánchez-Parra, Beatriz; Núñez, Andrés; Moreno, Diego A.

Publication:
Preventing legionellosis outbreaks by a quick detection of airborne Legionella pneumophila

dc.contributor.author	Sánchez-Parra, Beatriz
dc.contributor.author	Núñez, Andrés
dc.contributor.author	Moreno, Diego A.
dc.contributor.department	Genética y Microbiología
dc.date.accessioned	2024-07-22T08:50:06Z
dc.date.available	2024-07-22T08:50:06Z
dc.date.issued	2019-01-15
dc.description	© 2019 Elsevier Inc. All rights reserved. This document is the Published, version of a Published Work that appeared in final form in Environmental Research. To access the final edited and published work see https://doi.org/10.1016/j.envres.2019.01.032
dc.description.abstract	Legionellosis is a severe pneumonic infection caused by inhaling bacteria of the genus Legionella. Most cases reported in the USA and Europe are associated with the species Legionella pneumophila. This Gram-negative bacterium can survive within a wide spectrum of temperatures, and be transmitted via aerosols from multiple aquatic sources: fountains, thermal spas and other water systems. Although the PCR is one of the most popular methods to verify its presence in environmental or clinical samples, the direct application of this technique to ambient air samples is unusual because of the scarce material in the specimens. Here, we have developed a two-PCR assay, carried out over the V3 and V5 hypervariable regions of the 16S rRNA gene, to detect specifically the pathogenic bacteria Legionella pneumophila in outdoor air samples with low concentration of DNA. The application of this protocol does not require culture and retrieves quick results to activate the corresponding public alerts to prevent legionellosis outbreaks.	es
dc.format	application/pdf	es
dc.format.extent	4	es
dc.identifier.citation	Environmental research 171 (2019): 546-549
dc.identifier.doi	https://doi.org/10.1016/j.envres.2019.01.032
dc.identifier.issn	Print: 0013-9351
dc.identifier.uri	http://hdl.handle.net/10201/143306
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	Agencia financiadora: Comunidad de Madrid .Convocatoria: Convocatoria de Tecnologías 2013. Ámbito del proyecto: Regional. Nombre del proyecto: AIRBIOTA-CM: CONOCER Y MODELIZAR LA CONTAMINACIÓN BIOLÓGICA DEL AIRE URBANO. Código o número del acuerdo de subvención:S2013/MAE-2874.	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0013935119300325?via%3Dihub
dc.relation.requires	https://www.ncbi.nlm.nih.gov/nuccore/MH412932?report=genbank	es
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	Microbiology	es
dc.subject	Legionella	es
dc.subject	Bioaerosol	es
dc.subject	PCR	es
dc.subject	16S rRNA	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica	es
dc.title	Preventing legionellosis outbreaks by a quick detection of airborne Legionella pneumophila	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es